A Novel Hydrogen Peroxide Biosensor Based on Adsorption of Horseradish Peroxidase onto a Nanobiomaterial Composite Modified Glassy Carbon Electrode

被引：37

作者：

Moyo, Mambo ^{[1
]}

Okonkwo, Jonathan O. ^{[1
]}

Agyei, Nana M. ^{[2
]}

机构：

[1] Tshwane Univ Technol, Dept Environm Water & Earth Sci, ZA-0001 Pretoria, South Africa

[2] Univ Limpopo, Dept Chem, ZA-0204 Medunsa, South Africa

来源：

ELECTROANALYSIS | 2013年 / 25卷 / 08期

关键词：

Hydrogen peroxide; Adsorption; Horseradish peroxidase; Maize tassel; Multiwalled carbon nanotube; Glassy carbon electrode; DIRECT ELECTROCHEMISTRY; AMPEROMETRIC BIOSENSOR; NANOCOMPOSITE FILM; NAFION NANOCOMPOSITE; ELECTROCATALYSIS; NANOTUBES; GEL; BIOELECTROCATALYSIS; CHITOSAN; IMMOBILIZATION;

D O I：

10.1002/elan.201300165

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

In this work, a novel hydrogen peroxide biosensor derived from maize tassel (MT) and multiwalled carbon nanotube (MWCNT) composite was used to adsorb horseradish peroxidase (HRP) onto the surface of a glassy carbon electrode through electrostatic interactions. The morphology and structure of the products were characterized by SEM, FTIR and UV-visible spectroscopy. The electrochemical and electrocatalytic performance of the HRP/MT-MWCNT/GCE was studied using voltammetric and amperometric methods. The amperometric response of the biosensor varied linearly with concentration of H2O2 from 9 mu M to 1mM with detection limit of 4.0 mu M (S/N=3). Furthermore, the biosensor exhibited good reproducibility and stability.

引用

页码：1946 / 1954

页数：9

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