Endogenous and synthetic microRNAs stimulate simultaneous, efficient, and localized regulation of multiple targets in diverse species

被引:287
作者
Alvarez, JP
Pekker, I
Goldshmidt, A
Blum, E
Amsellem, Z
Eshed, Y [1 ]
机构
[1] Weizmann Inst Sci, Dept Plant Sci, IL-76100 Rehovot, Israel
[2] Monash Univ, Sch Biol Sci, Clayton, Vic 3800, Australia
关键词
D O I
10.1105/tpc.105.040725
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Recent studies demonstrated that pattern formation in plants involves regulation of transcription factor families by microRNAs (miRNAs). To explore the potency, autonomy, target range, and functional conservation of miRNA genes, a systematic comparison between plants ectopically expressing pre-miRNAs and plants with corresponding multiple mutant combinations of target genes was performed. We show that regulated expression of several Arabidopsis thaliana pre-miRNA genes induced a range of phenotypic alterations, the most extreme ones being a phenocopy of combined loss of their predicted target genes. This result indicates quantitative regulation by miRNA as a potential source for diversity in developmental outcomes. Remarkably, custom-made, synthetic miRNAs vectored by endogenous pre-miRNA backbones also produced phenocopies of multiple mutant combinations of genes that are not naturally regulated by miRNA. Arabidopsis-based endogenous and synthetic pre-miRNAs were also processed effectively in tomato (Solanum lycopersicum) and tobacco (Nicotiana tabacum). Synthetic miR-ARF targeting Auxin Response Factors 2, 3, and 4 induced dramatic transformations of abaxial tissues into adaxial ones in all three species, which could not cross graft joints. Likewise, organ-specific expression of miR165b that coregulates the PHABULOSA-like adaxial identity genes induced localized abaxial transformations. Thus, miRNAs provide a flexible, quantitative, and autonomous platform that can be employed for regulated expression of multiple related genes in diverse species.
引用
收藏
页码:1134 / 1151
页数:18
相关论文
共 70 条
[1]   Modulation of floral development by a gibberellin-regulated microRNA [J].
Achard, P ;
Herr, A ;
Baulcombe, DC ;
Harberd, NP .
DEVELOPMENT, 2004, 131 (14) :3357-3365
[2]   Genes involved in organ separation in Arabidopsis: An analysis of the cup-shaped cotyledon mutant [J].
Aida, M ;
Ishida, T ;
Fukaki, H ;
Fujisawa, H ;
Tasaka, M .
PLANT CELL, 1997, 9 (06) :841-857
[3]  
Allen E, 2005, CELL, V121, P207, DOI 10.1016/j.cell.2005.04.004
[4]  
[Anonymous], 1991, PLANT TISSUE CULT
[5]   Regulation of flowering time and floral organ identity by a microRNA and its APETALA2-like target genes [J].
Aukerman, MJ ;
Sakai, H .
PLANT CELL, 2003, 15 (11) :2730-2741
[6]   Antiquity of microRNAs and their targets in land plants [J].
Axtell, MJ ;
Bartel, DP .
PLANT CELL, 2005, 17 (06) :1658-1673
[7]   The early extra petals1 mutant uncovers a role for MicroRNA miR164c in regulating petal number in Arabidopsis [J].
Baker, CC ;
Sieber, P ;
Wellmer, F ;
Meyerowitz, EM .
CURRENT BIOLOGY, 2005, 15 (04) :303-315
[8]   MicroRNA binding sites in Arabidopsis class IIIHD-ZIP mRNAs are required for methylation of the template chromosome [J].
Bao, N ;
Lye, KW ;
Barton, MK .
DEVELOPMENTAL CELL, 2004, 7 (05) :653-662
[9]   MicroRNAs: At the root of plant development? [J].
Bartel, B ;
Bartel, DP .
PLANT PHYSIOLOGY, 2003, 132 (02) :709-717
[10]   MicroRNAs: Genomics, biogenesis, mechanism, and function (Reprinted from Cell, vol 116, pg 281-297, 2004) [J].
Bartel, David P. .
CELL, 2007, 131 (04) :11-29