Multiplexing of Y chromosome specific STRs and performance for mixed samples

被引:142
作者
Prinz, M
Boll, K
Baum, H
Shaler, B
机构
[1] Department of Forensic Biology, Office of the Chief Medical Examiner, New York
[2] Office of Chief Medical Examiner, New York, NY 10016
关键词
Y-chromosome; STR polymorphism; multiplex; mixed samples;
D O I
10.1016/S0379-0738(96)02096-8
中图分类号
DF [法律]; D9 [法律]; R [医药、卫生];
学科分类号
0301 ; 10 ;
摘要
A combination of four Y-specific polymorphic STR loci was amplified simultaneously using fluorescently labeled primers. Multiplex conditions required optimization to eliminate constant bands and amplification products for female DNA. A series of experiments was carried out for mixtures of DNA from two males, and from male and female individuals for the Y-specific STRs and an autosomal locus. For the male/male mixtures amplified with the Y specific system, and amplified for an autosomal locus; the minor component in the mixture could only be identified up to a ratio of 1:10, 1:50 respectively. In male/female DNA mixtures the Y STR alleles could be identified for the highest ratio tested, 400 pg male in DNA in 800 ng female DNA which amounts to a ratio of 1:2000. (C) 1997 Elsevier Science Ireland Ltd.
引用
收藏
页码:209 / 218
页数:10
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