Dynamics of the gut microbiota in rats after hypobaric hypoxia exposure

被引:7
作者
Han, Yang [1 ,2 ]
Xu, Jiayu [1 ]
Yan, Yan [1 ]
Zhao, Xiaojing [1 ,2 ]
机构
[1] Med Innovat Res Div Chinese PLA Gen Hosp, Translat Med Res Ctr, Beijing, Peoples R China
[2] Minist Ind & Informat Technol, Med Innovat Res Div Chinese PLA Gen Hosp, Key Lab Biomed Engn & Translat Med, Beijing, Peoples R China
来源
PEERJ | 2022年 / 10卷
基金
中国国家自然科学基金;
关键词
Gut microbiota; Dynamics; Hypobaric hypoxia; Normoxia; Anaerobes; ALIGNMENT;
D O I
10.7717/peerj.14090
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Background. Gut microbiota plays an important role in host health and is influenced by multiple factors. Hypobaric hypoxia usually existing at high altitude conditions can adversely affect normal physiological functions. However, the dynamic changes of gut microbiota influenced by hypobaric hypoxia have not been elucidated. Methods. In this study, we collected fecal samples from seven rats at 14 time points from entering the hypobaric chamber (eight time points) to leaving the chamber (six time points) and five rats served as normoxic controls. Metagenome sequencing was performed on all samples and the dynamics of taxa and functions were analyzed. Results. We found that the alpha-diversity was changed in the first 5 days after entering or leaving the hypobaric chamber. The beta-diversity analysis revealed that gut microbiota structure was significantly separated among 14 time points. After entering the chamber, the relative abundance of Bacteroides decreased and the most abundant genus turned into Prevotella. The abundance of Firmicutes and Bacteroidetes showed an opposite trend and both have a significant change within 5 days after entering or leaving the hypobaric hypoxia chamber. Some obligate anaerobic bacteria belonging to Desulfovibrio and Alistipes were significantly enriched after entering the chamber for 5 weeks, whereas Probiotics like Bifidobacterium and Lactococcus, and short-chain fatty acids producers like Butyrivibrio and Pseudobutyrivibrio were significantly enriched after leaving the chamber for 3 weeks. Microbial functions like 'Two-component regulatory system', 'beta-carotene biosynthesis' and 'Fatty acid biosynthesis' were significantly enriched after entering the chamber for 5 weeks. Hypobaric hypoxia conditions could deeply affect the diversity and structure of gut microbiota. The alterations of abundance of dominant taxa (Firmicutes and Bacteroidetes), increased anaerobes and decreased probiotics induced by hypobaric hypoxia conditions might affect the host health.
引用
收藏
页数:19
相关论文
共 58 条
[1]   Correlation Between Intraluminal Oxygen Gradient and Radial Partitioning of Intestinal Microbiota [J].
Albenberg, Lindsey ;
Esipova, Tatiana V. ;
Judge, Colleen P. ;
Bittinger, Kyle ;
Chen, Jun ;
Laughlin, Alice ;
Grunberg, Stephanie ;
Baldassano, Robert N. ;
Lewis, James D. ;
Li, Hongzhe ;
Thom, Stephen R. ;
Bushman, Frederic D. ;
Vinogradov, Sergei A. ;
Wu, Gary D. .
GASTROENTEROLOGY, 2014, 147 (05) :1055-+
[2]   Fatty Acid Biosynthesis Inhibition Increases Reduction Potential in Neuronal Cells under Hypoxia [J].
Brose, Stephen A. ;
Golovko, Svetlana A. ;
Golovko, Mikhail Y. .
FRONTIERS IN NEUROSCIENCE, 2016, 10
[3]   Fast and sensitive protein alignment using DIAMOND [J].
Buchfink, Benjamin ;
Xie, Chao ;
Huson, Daniel H. .
NATURE METHODS, 2015, 12 (01) :59-60
[4]   Cardiopulmonary and metabolic responses in healthy elderly humans during a 1-week hiking programme at high altitude [J].
Burtscher, M ;
Bachmann, O ;
Hatzl, T ;
Hotter, B ;
Likar, R ;
Philadelphy, M ;
Nachbauer, W .
EUROPEAN JOURNAL OF APPLIED PHYSIOLOGY, 2001, 84 (05) :379-386
[5]   Metagenome-wide association analysis identifies microbial determinants of post-antibiotic ecological recovery in the gut [J].
Chng, Kern Rei ;
Ghosh, Tarini Shankar ;
Tan, Yi Han ;
Nandi, Tannistha ;
Lee, Ivor Russel ;
Ng, Amanda Hui Qi ;
Li, Chenhao ;
Ravikrishnan, Aarthi ;
Lim, Kar Mun ;
Lye, David ;
Barkham, Timothy ;
Raman, Karthik ;
Chen, Swaine L. ;
Chai, Louis ;
Young, Barnaby ;
Gan, Yunn-Hwen ;
Nagarajan, Niranjan .
NATURE ECOLOGY & EVOLUTION, 2020, 4 (09) :1256-1267
[6]   Anaerophaga thermohalophila gen. nov., sp nov., a moderately thermohalophilic, strictly anaerobic fermentative bacterium [J].
Denger, K ;
Warthmann, R ;
Ludwig, W ;
Schink, B .
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2002, 52 :173-178
[7]   Gut microbiota in human metabolic health and disease [J].
Fan, Yong ;
Pedersen, Oluf .
NATURE REVIEWS MICROBIOLOGY, 2021, 19 (01) :55-71
[8]  
Fanaro S, 2003, ACTA PAEDIATR, V92, P48
[9]   Molecular monitoring of succession of bacterial communities in human neonates [J].
Favier, CF ;
Vaughan, EE ;
De Vos, WM ;
Akkermans, ADL .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2002, 68 (01) :219-226
[10]   Time series analysis of microbiome and metabolome at multiple body sites in steady long-term isolation confinement [J].
Feng, Qiang ;
Lan, Xiang ;
Ji, Xiaoli ;
Li, Meihui ;
Liu, Shili ;
Xiong, Jianghui ;
Yu, Yanbo ;
Liu, Zhipeng ;
Xu, Zi ;
He, Li ;
Chen, Ying ;
Dong, Haisheng ;
Chen, Pu ;
Chen, Bin ;
He, Kunlun ;
Li, Yinghui .
GUT, 2021, 70 (07) :1409-+