Fluorescence lifetime spectroscopy of tissue autofluorescence in normal and diseased colon measured ex vivo using a fiber-optic probe

被引：53
作者：
Coda, Sergio [1 ,2 ]
Thompson, Alex J. [1 ]
Kennedy, Gordon T. [1 ]
Roche, Kim L. [1 ,2 ]
Ayaru, Lakshmana [2 ]
Bansi, Devinder S. [2 ]
Stamp, Gordon W. [3 ,4 ]
Thillainayagam, Andrew V. [1 ,2 ]
French, Paul M. W. [1 ]
Dunsby, Chris [1 ,3 ]
机构：
[1] Univ London Imperial Coll Sci Technol & Med, Dept Phys, Photon Grp, London SW7 2AZ, England
[2] Imperial Coll Healthcare NHS Trust, Charing Cross Hosp, Dept Gastroenterol, Endoscopy Unit, London W6 8RF, England
[3] Univ London Imperial Coll Sci Technol & Med, Dept Histopathol, London W12 0NN, England
[4] Canc Res UK London Res Inst, London WC2A 3LY, England
来源：
BIOMEDICAL OPTICS EXPRESS | 2014年 / 5卷 / 02期
基金：
英国工程与自然科学研究理事会; 英国生物技术与生命科学研究理事会;
关键词：
LASER-INDUCED FLUORESCENCE; LIGHT-SCATTERING SPECTROSCOPY; LAGUERRE EXPANSION TECHNIQUE; HIGH-RESOLUTION ENDOSCOPY; HIGH-GRADE DYSPLASIA; IN-VIVO; BARRETTS-ESOPHAGUS; DIFFUSE-REFLECTANCE; MULTIPHOTON MICROSCOPY; EARLY NEOPLASIA;
D O I：
10.1364/BOE.5.000515
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
We present an ex vivo study of temporally and spectrally resolved autofluorescence in a total of 47 endoscopic excision biopsy/resection specimens from colon, using pulsed excitation laser sources operating at wavelengths of 375 nm and 435 nm. A paired analysis of normal and neoplastic (adenomatous polyp) tissue specimens obtained from the same patient yielded a significant difference in the mean spectrally averaged autofluorescence lifetime -570 +/- 740 ps (p = 0.021, n = 12). We also investigated the fluorescence signature of non-neoplastic polyps (n = 6) and inflammatory bowel disease (n = 4) compared to normal tissue in a small number of specimens. (C) 201 Optical Society of America
引用
收藏
页码：515 / 538
页数：24
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