Anti-inflammatory activity of lansais from endophytic Streptomyces sp SUC1 in LPS-induced RAW 264.7 cells

被引：19

作者：

Taechowisan, Thongchai ^{[1
]}

Wanbanjob, Asawin ^{[2
]}

Tuntiwachwuttikul, Pittaya ^{[2
]}

Liu, Jikai ^{[3
]}

机构：

[1] Silpakorn Univ, Dept Microbiol, Nakhon Pathom 73000, Thailand

[2] Silpakorn Univ, Dept Chem, Nakhon Pathom 73000, Thailand

[3] Chinese Acad Sci, KIB, Kunming 650204, Peoples R China

来源：

FOOD AND AGRICULTURAL IMMUNOLOGY | 2009年 / 20卷 / 01期

关键词：

anti-inflammatory activity; endophytic Streptomyces; lansai C; RAW; 264; 7; cells; NITRIC-OXIDE PRODUCTION; MACROPHAGES; LIPOPOLYSACCHARIDE; CYCLOOXYGENASE-2; INFLAMMATION; CYTOKINES; PAIN;

D O I：

10.1080/09540100902730064

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

This research was undertaken to find the in vitro inflammatory action of lansai A-D produced by Streptomyces sp. SUC1. We investigated the effects of lansai C not only on the formation of nitric oxide (NO), prostaglandin E2 (PGE2), tumour necrosis factor (TNF-), interleukin (IL)-1, IL-6 and IL-10, but also on inducible NO synthase and cyclooxygenase-2 (COX-2) in lipopolysaccharide (LPS)-induced murine macrophage RAW 264.7 cells. The data obtained were consistent with the modulation of inducible nitric oxide synthase enzyme expression. A similar fashion was also observed when LPS-induced PGE2 release and COX-2 expression were tested. The significant inhibitory effects were shown in concentration-dependent manners. In addition, lansai C also mildly but significantly reduced the formation of TNF-. These findings support the application of lansai C as anti-inflammatory agent.

引用

页码：67 / 77

页数：11

共 21 条

[1] RAPID DIFFERENTIATION BETWEEN NOCARDIA + STREPTOMYCES BY PAPER CHROMATOGRAPHY OF WHOLE-CELL HYDROLYSATES [J].

BECKER, B ;

GORDON, RE ;

LECHEVALIER, HA ;

LECHEVALIER, MP .

APPLIED MICROBIOLOGY, 1964, 12 (05) :421-+

[2] RAPID METHOD FOR CHARACTERIZATION OF ACTINOMYCETES BY CELL WALL COMPOSITION [J].

BOONE, CJ ;

PINE, L .

APPLIED MICROBIOLOGY, 1968, 16 (02) :279-+

[3] Modulation of cytokine secretion by garlic oil derivatives is associated with suppressed nitric oxide production in stimulated macrophages [J].

Chang, HP ;

Huang, SY ;

Chen, YH .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2005, 53 (07) :2530-2534

[4] Role of cytokines in rheumatoid arthritis [J].

Feldmann, M ;

Brennan, FM ;

Maini, RN .

ANNUAL REVIEW OF IMMUNOLOGY, 1996, 14 :397-440

[5] Isoproterenol inhibits IL-10, TNF-α, and nitric oxide production in RAW 264.7 macrophages [J].

Haskó, G ;

Németh, ZH ;

Szabó, C ;

Zsilla, G ;

Salzman, AL ;

Vizi, ES .

BRAIN RESEARCH BULLETIN, 1998, 45 (02) :183-187

[6]

LEE SH, 1992, J BIOL CHEM, V267, P25934

[7] SELECTIVE-INHIBITION OF INDUCIBLE CYCLOOXYGENASE-2 IN-VIVO IS ANTIINFLAMMATORY AND NONULCEROGENIC [J].

MASFERRER, JL ;

ZWEIFEL, BS ;

MANNING, PT ;

HAUSER, SD ;

LEAHY, KM ;

SMITH, WG ;

ISAKSON, PC ;

SEIBERT, K .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (08) :3228-3232

[8]

MONCADA S, 1993, NEW ENGL J MED, V329, P2002

[9] RAPID COLORIMETRIC ASSAY FOR CELLULAR GROWTH AND SURVIVAL - APPLICATION TO PROLIFERATION AND CYTO-TOXICITY ASSAYS [J].

MOSMANN, T .

JOURNAL OF IMMUNOLOGICAL METHODS, 1983, 65 (1-2) :55-63

[10] Targeting NOX, INOS and COX-2 in inflammatory cells: Chemoprevention using food phytochemicals [J].

Murakami, Akira ;

Ohigashi, Hajime .

INTERNATIONAL JOURNAL OF CANCER, 2007, 121 (11) :2357-2363

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