MicroRNA-197 influences 5-fluorouracil resistance via thymidylate synthase in colorectal cancer

被引：36

作者：

Sun, Z. ^{[1
,2
]}

Zhou, N. ^{[1
,2
]}

Han, Q. ^{[3
]}

Zhao, L. ^{[1
,2
]}

Bai, C. ^{[1
,2
]}

Chen, Y. ^{[2
,4
]}

Zhou, J. ^{[1
,2
]}

Zhao, R. C. ^{[3
,5
]}

机构：

[1] Chinese Acad Med Sci, Peking Union Med Coll Hosp, Dept Oncol, Beijing 100730, Peoples R China

[2] Peking Union Med Coll, Beijing 100021, Peoples R China

[3] Chinese Acad Med Sci, Inst Basic Med Sci, Sch Basic Med, Peking Union Med Coll, Beijing 100730, Peoples R China

[4] Chinese Acad Med Sci, Dept Digest, Peking Union Med Coll Hosp, Beijing 100730, Peoples R China

[5] Peking Union Med Coll Hosp, Ctr Translat Med, Beijing, Peoples R China

来源：

CLINICAL & TRANSLATIONAL ONCOLOGY | 2015年 / 17卷 / 11期

基金：

中国国家自然科学基金;

关键词：

miR-197; 5-Fluorouracil; Resistance; Thymidylate synthase; Colorectal cancer; EXPRESSION PROFILE; CELLS; CHEMOSENSITIVITY; IDENTIFICATION; CHEMOTHERAPY; SENSITIVITY;

D O I：

10.1007/s12094-015-1318-7

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The response rate of first-line fluoropyrimidine-based regimens for metastatic colorectal cancer (mCRC) is generally less than 50 %. The down-regulation of miR-197 in colorectal cancer cells after exposure to 5-fluorouracil might be related to the mechanism of resistance to fluoropyrimidine-based chemotherapy. So we investigated the regulatory mechanism of miR-197 on 5-FU sensitivity. Dual luciferase reporter gene construct and dual luciferase reporter assay were used to identify the target of miR-197. TYMS expression was evaluated by immunohistochemistry staining. 5-Fu resistance of colorectal cancer cell lines was detected by MTS assay. The expression of miR-197 was detected by real time PCR. A luciferase assay and western blot analysis confirmed that miR-197 directly binds to and negatively regulates TYMS expression. Overexpressing miR-197 could increase the sensitivity of colorectal cancer cells to 5-fluorouracil (5-FU). The expression of miR-197 negatively correlated with TYMS expression in cancerous tissues from patients with stage IV colorectal cancer. miR-197 mediates the response of colorectal cancer cells to 5-FU by regulating TYMS expression.

引用

页码：876 / 883

页数：8

共 26 条

[21]

Siegel RL, 2020, ANTI-CANCER DRUG, V70, P7, DOI [10.3322/caac.21590, DOI 10.1097/CAD.0000000000000617]

[22] The Clinical Significance of MiR-148a as a Predictive Biomarker in Patients with Advanced Colorectal Cancer [J].