Development of low cost medium for ethanol production from syngas by Clostridium ragsdalei

被引：50

作者：

Gao, Jie ^{[1
]}

Atiyeh, Hasan K. ^{[1
]}

Phillips, John R. ^{[1
]}

Wilkins, Mark R. ^{[1
]}

Huhnke, Raymond L. ^{[1
]}

机构：

[1] Oklahoma State Univ, Dept Biosyst & Agr Engn, Stillwater, OK 74078 USA

来源：

BIORESOURCE TECHNOLOGY | 2013年 / 147卷

关键词：

Syngas fermentation; Clostridium ragsdalei; Ethanol; Medium development; Defined medium; SYNTHESIS GAS FERMENTATION; BIOLOGICAL PRODUCTION; PRICE VOLATILITY; CARBOXIDIVORANS; SOLVENTS; EXTRACT; GROWTH;

D O I：

10.1016/j.biortech.2013.08.075

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

The development of a low cost medium for ethanol production is critical for process feasibility. Ten media were formulated for Clostridium ragsdalei by reduction, elimination and replacement of expensive nutrients. Cost analysis and effects of medium components on growth and product formation were investigated. Fermentations were performed in 250 mL bottles using syngas (20% CO, 15% CO2, 5% H-2 and 60% N-2). The standard medium M1 cost is $9.83/L, of which 93% is attributed to morpholinoethane sulfonic acid (MES) buffer. Statistical analysis of the results showed that MES removal did not affect cell growth and ethanol production (P>0.05). Based on cells' elemental composition, a minimal mineral concentration medium M7 was formulated, which provided 29% higher ethanol yield from CO at 3% of the cost compared to medium M1. Ethanol yield from CO in the completely defined medium M9 was 36% higher than while at 5% the cost of medium M1. (C) 2013 Elsevier Ltd. All rights reserved.

引用

页码：508 / 515

页数：8

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