Overexpression of Arabidopsis YUCCA6 in Potato Results in High-Auxin Developmental Phenotypes and Enhanced Resistance to Water Deficit

被引:157
作者
Kim, Jeong Im [1 ]
Baek, Dongwon [2 ,3 ]
Park, Hyeong Cheol [2 ,3 ]
Chun, Hyun Jin [2 ,3 ]
Oh, Dong-Ha [4 ]
Lee, Min Kyung [5 ]
Cha, Joon-Yung [2 ,3 ]
Kim, Woe-Yeon [2 ,3 ]
Kim, Min Chul [2 ,3 ]
Chung, Woo Sik [2 ,3 ]
Bohnert, Hans J. [4 ,6 ]
Lee, Sang Yeol [2 ,3 ]
Bressan, Ray A. [6 ,7 ]
Lee, Shin-Woo [5 ]
Yun, Dae-Jin [2 ,3 ]
机构
[1] Purdue Univ, Dept Biochem, W Lafayette, IN 47907 USA
[2] Gyeongsang Natl Univ, Plant Mol Biol & Biotechnol Res Ctr, Div Appl Life Sci, BK21, Jinju 660701, South Korea
[3] Gyeongsang Natl Univ, Plant Mol Biol & Biotechnol Res Ctr, WCU Program, Jinju 660701, South Korea
[4] Univ Illinois, Dept Plant Biol, Urbana, IL USA
[5] Gyeongnam Natl Univ Sci & Technol, Dept Agron & Med Plant Resources, Jinju 660758, South Korea
[6] King Abdulaziz Univ, Fac Sci, Jeddah 21589, Saudi Arabia
[7] Purdue Univ, Dept Hort, W Lafayette, IN 47907 USA
基金
新加坡国家研究基金会;
关键词
Solanum tuberosum; potato; Arabidopsis thaliana; yuc6-1D; YUCCA; StYUCCA; StTAA; auxin; drought; YUCCA FLAVIN MONOOXYGENASES; REPRODUCTIVE DEVELOPMENT; DROUGHT-STRESS; BIOSYNTHESIS; TRYPTOPHAN; GENE; PATHWAY; ACID; LEAF; PLANTS;
D O I
10.1093/mp/sss100
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Indole-3-acetic acid (IAA), a major plant auxin, is produced in both tryptophan-dependent and tryptophan-independent pathways. A major pathway in Arabidopsis thaliana generates IAA in two reactions from tryptophan. Step one converts tryptophan to indole-3-pyruvic acid (IPA) by tryptophan aminotransferases followed by a rate-limiting step converting IPA to IAA catalyzed by YUCCA proteins. We identified eight putative StYUC (Solanum tuberosum YUCCA) genes whose deduced amino acid sequences share 50%70% identity with those of Arabidopsis YUCCA proteins. All include canonical, conserved YUCCA sequences: FATGY motif, FMO signature sequence, and FAD-binding and NADP-binding sequences. In addition, five genes were found with similar to 50% amino acid sequence identity to Arabidopsis tryptophan aminotransferases. Transgenic potato (Solanum tuberosum cv. Jowon) constitutively overexpressing Arabidopsis AtYUC6 displayed high-auxin phenotypes such as narrow downward-curled leaves, increased height, erect stature, and longevity. Transgenic potato plants overexpressing AtYUC6 showed enhanced drought tolerance based on reduced water loss. The phenotype was correlated with reduced levels of reactive oxygen species in leaves. The results suggest a functional YUCCA pathway of auxin biosynthesis in potato that may be exploited to alter plant responses to the environment.
引用
收藏
页码:337 / 349
页数:13
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