The microRNA miR-132 targets Lrrfip1 to block vascular smooth muscle cell proliferation and neointimal hyperplasia

被引：91

作者：

Choe, Nakwon ^{[1
,2
]}

Kwon, Jin-Sook ^{[3
]}

Kim, Ju-Ryoung ^{[1
,2
]}

Eom, Gwang Hyeon ^{[1
,2
]}

Kim, Yongsook ^{[3
]}

Nam, Kwang-Il ^{[2
,4
]}

Ahn, Youngkeun ^{[3
]}

Kee, Hae Jin ^{[3
]}

Kook, Hyun ^{[1
,2
]}

机构：

[1] Chonnam Natl Univ, Sch Med, Dept Pharmacol, Med Res Ctr Gene Regulat, Kwangju 501746, South Korea

[2] Chonnam Natl Univ, Sch Med, Natl Res Lab Heart & Muscle Dis, Kwangju 501746, South Korea

[3] Chonnam Natl Univ Hosp, Heart Res Ctr, Kwangju 501746, South Korea

[4] Chonnam Natl Univ, Sch Med, Dept Anat, Med Res Ctr Gene Regulat, Kwangju 501746, South Korea

来源：

ATHEROSCLEROSIS | 2013年 / 229卷 / 02期

基金：

新加坡国家研究基金会;

关键词：

miR-132; Leucine-rich repeat (in flightless 1); interacting protein-1; Vascular smooth muscle cells; Neointimal hyperplasia; Atherosclerosis; LEUCINE-RICH REPEAT; ARTERIAL INJURY; GENE-EXPRESSION; FLIGHTLESS I; PROTEIN; GROWTH; TRANSCRIPTION; ACTIVATION; PLASTICITY; PHENOTYPE;

D O I：

10.1016/j.atherosclerosis.2013.05.009

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Objective: The proliferation and remodeling of vascular smooth muscle cells (VSMCs) is an important pathological event in atherosclerosis and restenosis. Here we report that microRNA-132 (miR-132) blocks vascular smooth muscle cells (VSMC) proliferation by inhibiting the expression of LRRFIP1 [leucine-rich repeat (in Flightless 1) interacting protein-1]. Methods and results: MicroRNA microarray revealed that miR-132 was upregulated in the rat carotid artery after catheter injury, which was further confirmed by quantitative real-time RT-PCR. Transfection of a miR-132 mimic significantly inhibited the proliferation of VSMCs, whereas transfection of a miR-132 antagomir increased it. miR-132 mimic inhibited VSMC migration and induced apoptosis. miR-132 mimic increased the protein amounts of both p27 and smooth muscle (SM) alpha-actin, whereas it decreased SM alpha-actin and Bcl2. Bioinformatics showed that LRRFIP1 is a target candidate of miR-132. miR-132 downregulated luciferase activity driven by a vector containing the 30-untranslated region of Lrrfip1 in a sequence-specific manner. LRRFIP1 induced VSMC proliferation and increased phosphorylation of ERK. Immunohistochemical analysis revealed that Lrrfip1 was clearly expressed along with the basal laminar area of smooth muscle, and its expression pattern was disrupted 7 days after arterial injury. LRRFIP1 mRNA was decreased 14 days after injury. Delivery of miR-132 to rat carotid artery reduced LRRFIP1 expression and attenuated neointimal proliferation in carotid artery injury models. Conclusions: Our results suggest that miR-132 is a novel regulator of VSMC proliferation that represses neointimal formation by inhibiting LRRFIP1 expression. (C) 2013 Elsevier Ireland Ltd. All rights reserved.

引用

页码：348 / 355

页数：8