Development of a disposable pyruvate biosensor to determine pungency in onions (Allium cepa L.)

被引:31
作者
Abayomi, LA [1 ]
Terry, LA [1 ]
White, SF [1 ]
Warner, PJ [1 ]
机构
[1] Cranfield Univ, Plant Sci Lab, Bedford MK45 4DT, England
关键词
meldolas blue; fresh produce; quality assurance; sweet onions;
D O I
10.1016/j.bios.2005.10.024
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
A disposable prototype pyruvate biosensor was constructed using pyruvate oxidase immobilised on mediated meldolas blue electrodes to determine pungency in onions (Allium cepa L.). The optimum operating potential was +150mV (versus Ag/AgCl). A strong correlation between the biosensor response and untreated onion juice of known pyruvate concentration 2-12 mu mol/g fresh weight (FW) was demonstrated. The biosensor was able to differentiate between low and high pungency onions. The detection limit using 1 unit of pyruvate oxidase was 1-2 mu mol/g FW. Optimum concentrations of co-factors TPP, FAD and MgSO4 comprising the enzyme cocktail were determined as being 0.04, 0.1 and 30 mm, respectively. (c) 2005 Elsevier B.V. All rights reserved.
引用
收藏
页码:2176 / 2179
页数:4
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