Characterization of Bacillus halodurans α-galactosidase Mel4A encoded by the mel4A gene (BH2228)

被引：11

作者：

Anggraeni, Andian Ari ^{[1
]}

Sakka, Makiko ^{[1
]}

Kimura, Tetsuya ^{[1
]}

Ratanakhaokchai, Khanok ^{[2
]}

Kitaoka, Motomitsu ^{[3
]}

Sakka, Kazuo ^{[1
]}

机构：

[1] Mie Univ, Grad Sch Bioresources, Appl Microbiol Lab, Tsu, Mie 5148507, Japan

[2] King Mongkuts Univ Technol Thonburi, Sch Bioresources & Technol, Bangkok 10140, Thailand

[3] Natl Food Res Inst, Tsukuba, Ibaraki 3058642, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2008年 / 72卷 / 09期

基金：

日本科学技术振兴机构;

关键词：

Bacillus halodurans; alpha-galactosidase; melibiase; glycoside hydrolase; raffinose;

D O I：

10.1271/bbb.80242

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A family-4 alpha-galactosidase Mel4A of Bacillus halodurans was expressed in Escherichia coli and characterized. Recombinant enzyme rMel4A depended on NAD(+), some divalent cations such as Mn2(+), and reducing reagents such as dithiothreitol. rMel4A was active on small saccharides such as raffinose but not on highly polymerized galactomannan. Immunological analysis indicated that raffinose induced the production of Mel4A in B. halodurans.

引用

页码：2459 / 2462

页数：4

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