Targeted chimera delivery to ovarian cancer cells by heterogeneous gold magnetic nanoparticle

被引：31

作者：

Chen, Yao ^{[1
,5
]}

Xu, Mengjiao ^{[1
]}

Guo, Yi ^{[1
]}

Tu, Keyao ^{[2
]}

Wu, Weimin ^{[1
]}

Wang, Jianjun ^{[1
]}

Tong, Xiaowen ^{[1
]}

Wu, Wenjuan ^{[3
]}

Qi, Lifeng ^{[2
,6
]}

Shi, Donglu ^{[2
,4
]}

机构：

[1] Tongji Univ, Sch Med, Dept Obstet & Gynaecol, Tongji Hosp, Shanghai 200065, Peoples R China

[2] Tongji Univ, Sch Med, Inst Translat Nanomed, Shanghai East Hosp,Inst Biomed Engn & Nano Sci, Shanghai 200092, Peoples R China

[3] Tongji Univ, Sch Med, Shanghai East Hosp, Dept Lab Med, Shanghai 200123, Peoples R China

[4] Univ Cincinnati, Dept Mech & Mat Engn, Coll Engn & Appl Sci, Mat Sci & Engn Program, Cincinnati, OH 45221 USA

[5] Nanjing Med Univ, Affiliated Hosp 2, 121 Jiangjiayuan Rd, Nanjing 210011, Jiangsu, Peoples R China

[6] Shanghai Foxgene Med Co LTD, Room 501,Fudan Sci Technol Pk,11 Guotai Rd, Shanghai 200092, Peoples R China

来源：

NANOTECHNOLOGY | 2017年 / 28卷 / 02期

关键词：

chimera; Notch3; Au-Fe3O4; nanoparticles; multi-drug resistance; ovarian cancer; APTAMER-SIRNA CHIMERAS; ELONGATION-FACTOR; 2; IN-VITRO; GENE; INHIBITION; NOTCH3; GROWTH; ANGIOGENESIS; APOPTOSIS; VEGF(165);

D O I：

10.1088/0957-4484/28/2/025101

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

Efficient delivery of small interfering RNAs (siRNAs) to the targeted cells has remained a significant challenge in clinical applications. In the present study, we developed a novel aptamer-siRNA chimera delivery system mediated by cationic Au-Fe3O4 nanoparticles (NPs). The chimera constructed by VEGF RNA aptamer and Notch3 siRNA was bonded with heterogeneous Au-Fe3O4 nanoparticles by electrostatic interaction. The obtained complex exhibited much higher silencing efficiency against Notch3 gene compared with chimera alone and lipofectamine-siRNA complex, and improved the antitumor effects of the loaded chimera. Moreover, the efficient delivery of the chimera by Au-Fe3O4 NPs could reverse multi-drug resistance (MDR) of ovarian cancer cells against the chemotherapeutic drug cisplatin, indicating its potential capability for future targeted cancer therapy while overcoming MDR.

引用

页数：13

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