Identification and characterization of IS1476, an insertion sequence-like element that disrupts VanY function in a vancomycin-resistant Enterococcus faecium strain

被引：36
作者：
MacKinnon, MG
Drebot, MA
Tyrrell, GJ
机构：
[1] DALHOUSIE UNIV,DEPT MICROBIOL & IMMUNOL,HALIFAX,NS B3H 2Y9,CANADA
[2] QEII HLTH SCI CTR,NATL CTR ENTEROVIRUSES,HALIFAX,NS B3H 2Y9,CANADA
[3] QEII HLTH SCI CTR,DEPT PATHOL & LAB MED,DIV MICROBIOL,HALIFAX,NS B3H 2Y9,CANADA
[4] QEII HLTH SCI CTR,DEPT MED,HALIFAX,NS B3H 2Y9,CANADA
来源：
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 1997年 / 41卷 / 08期
关键词：
D O I：
10.1128/AAC.41.8.1805
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
The vanY gene of vancomycin-resistant enterococci encodes a D,D-carboxypeptidase. By using a PCR detection strategy, a VanA Enterococcus faecium clinical isolate was found to have an insertion sequence (IS)-like element designated IS/476 in vanY. The activity of the VanY D,D-carboxypeptidase in this isolate was decreased in a fluorometric fluoraldehyde o-phthalaldehyde assay with diacetyl-Lys-D-Ala-D-Ala as the substrate. This, to our knowledge, is the first report of an IS-like element in a vancomycin resistance gene.
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页码：1805 / 1807
页数：3
相关论文
共 16 条
[1]  
ALTSCHUL SF, 1990, J MOL BIOL, V215, P403, DOI 10.1006/jmbi.1990.9999
[2]   The VanS sensor negatively controls VanR-mediated transcriptional activation of glycopeptide resistance genes of Tn1546 and related elements in the absence of induction [J].
Arthur, M ;
Depardieu, F ;
Gerbaud, G ;
Galimand, M ;
Leclercq, R ;
Courvalin, P .
JOURNAL OF BACTERIOLOGY, 1997, 179 (01) :97-106
[3]   Quantitative analysis of the metabolism of soluble cytoplasmic peptidoglycan precursors of glycopeptide-resistant enterococci [J].
Arthur, M ;
Depardieu, F ;
Reynolds, P ;
Courvalin, P .
MOLECULAR MICROBIOLOGY, 1996, 21 (01) :33-44
[4]   SEQUENCE OF THE VANY GENE REQUIRED FOR PRODUCTION OF A VANCOMYCIN-INDUCIBLE D,D-CARBOXYPEPTIDASE IN ENTEROCOCCUS-FAECIUM BM4147 [J].
ARTHUR, M ;
MOLINAS, C ;
COURVALIN, P .
GENE, 1992, 120 (01) :111-114
[5]   CHARACTERIZATION OF TN1546, A TN3-RELATED TRANSPOSON CONFERRING GLYCOPEPTIDE RESISTANCE BY SYNTHESIS OF DEPSIPEPTIDE PEPTIDOGLYCAN PRECURSORS IN ENTEROCOCCUS-FAECIUM BM4147 [J].
ARTHUR, M ;
MOLINAS, C ;
DEPARDIEU, F ;
COURVALIN, P .
JOURNAL OF BACTERIOLOGY, 1993, 175 (01) :117-127
[6]   ISOLATION AND CHARACTERIZATION OF IS1181, AN INSERTION-SEQUENCE FROM STAPHYLOCOCCUS-AUREUS [J].
DERBISE, A ;
DYKE, KGH ;
ELSOLH, N .
PLASMID, 1994, 31 (03) :251-264
[7]   A NEW MOBILE GENETIC ELEMENT IN LACTOBACILLUS-DELBRUECKII SUBSP BULGARICUS [J].
GERMOND, JE ;
LAPIERRE, L ;
DELLEY, M ;
MOLLET, B .
MOLECULAR & GENERAL GENETICS, 1995, 248 (04) :407-416
[8]   VANCOMYCIN RESISTANCE IS ENCODED ON A PHEROMONE RESPONSE PLASMID IN ENTEROCOCCUS-FAECIUM 228 [J].
HANDWERGER, S ;
PUCCI, MJ ;
KOLOKATHIS, A .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1990, 34 (02) :358-360
[9]   IDENTIFICATION OF CHROMOSOMAL MOBILE ELEMENT CONFERRING HIGH-LEVEL VANCOMYCIN RESISTANCE IN ENTEROCOCCUS-FAECIUM [J].
HANDWERGER, S ;
SKOBLE, J .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1995, 39 (11) :2446-2453
[10]   HETEROGENEITY OF THE VANA GENE-CLUSTER IN CLINICAL ISOLATES OF ENTEROCOCCI FROM THE NORTHEASTERN UNITED-STATES [J].
HANDWERGER, S ;
SKOBLE, J ;
DISCOTTO, LF ;
PUCCI, MJ .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1995, 39 (02) :362-368
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