NeuroExaminer: an all-glass microfluidic device for whole-brain in vivo imaging in zebrafish

While microfluidics enables chemical stimuli application with high spatio-temporal precision, light-sheet microscopy allows rapid imaging of entire zebrafish brains with cellular resolution. Both techniques, however, have not been combined to monitor whole-brain neural activity yet. Unlike conventional microfluidics, we report here an all-glass device (NeuroExaminer) that is compatible with whole-brain in vivo imaging using light-sheet microscopy and can thus provide insights into brain function in health and disease. Kai Mattern, Jakob W. von Trotha, et al. develop NeuroExaminer, an all glass device for whole-brain in vivo imaging in zebrafish. The method is based on light-sheet microscopy and microfluidics and provides insights on brain function in live zebrafish.