Neuroprotective effects of progesterone in traumatic brain injury: blunted in vivo neutrophil activation at the blood-brain barrier

被引：42

作者：

Pascual, Jose L. ^{[1
]}

Murcy, Mohammad A. ^{[1
]}

Li, Shenghui ^{[2
]}

Gong, Wanfeng ^{[1
]}

Eisenstadt, Rachel ^{[1
]}

Kumasaka, Kenichiro ^{[1
]}

Sims, Carrie ^{[1
]}

Smith, Douglas H. ^{[3
]}

Browne, Kevin ^{[3
]}

Allen, Steve ^{[1
]}

Baren, Jill ^{[4
]}

机构：

[1] Univ Penn, Div Traumatol Surg Crit Care & Emergency Surg, Dept Surg, Perelman Sch Med,Trauma Ctr Penn, Philadelphia, PA 19104 USA

[2] Tianjin Med Univ, Tianjin Neurol Inst, Dept Neurosurg, Tianjin, Peoples R China

[3] Univ Penn, Dept Neurosurg, Ctr Brain Injury & Repair, Perelman Sch Med, Philadelphia, PA 19104 USA

[4] Univ Penn, Dept Emergency Med, Perelman Sch Med, Philadelphia, PA 19104 USA

来源：

AMERICAN JOURNAL OF SURGERY | 2013年 / 206卷 / 06期

关键词：

Intravital microscopy; Neutrophil; Endothelium; Blood-brain barrier; Traumatic brain injury; Progesterone; HYPERTONIC SALINE; INFLAMMATION; HORMONES; SODIUM; EDEMA;

D O I：

10.1016/j.amjsurg.2013.07.016

中图分类号：

R61 [外科手术学];

学科分类号：

摘要：

BACKGROUND: Progesterone (PRO) may confer a survival advantage in traumatic brain injury (TBI) by reducing cerebral edema. We hypothesized that PRO reduces edema by blocking polymorphonuclear (PMN) interactions with endothelium (EC) in the blood-brain barrier (BBB). METHODS: CD1 mice received repeated PRO (16 mg/kg intraperitoneally) or vehicle (cyclodextrin) for 36 hours after TBI. Sham animals underwent craniotomy without TBI. The modified Neurological Severity Score graded neurologic recovery. A second craniotomy allowed in vivo observation of pial EC/PMN interactions and vascular macromolecule leakage. Wet/dry ratios assessed cerebral edema. RESULTS: Compared with the vehicle, PRO reduced subjective cerebral swelling (2.9 +/- .1 vs 1.2 +/- .1, P < .001), PMN rolling (95 +/- 1.8 vs 57 +/- 2.0 cells/100 mu m/min P < .001), total EC/PMN adhesion (2.0 +/- .4 vs .8 +/- .1 PMN/100 mu m, P < .01), and vascular permeability (51.8% +/- 4.9% vs 27.1% +/- 4.6%, P < .01). TBI groups had similar a Neurological Severity Score and cerebral wet/dry ratios (P > .05). CONCLUSIONS: PRO reduces live pericontusional EC/PMN and BBB macromolecular leakage after TBI. Direct PRO effects on the microcirculation warrant further investigation. (C) 2013 Elsevier Inc. All rights reserved.

引用

页码：840 / 845

页数：6

共 26 条

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