Capsaicin production by Alternaria alternata, an endophytic fungus from Capsicum annum, LC-ESI-MS/MS analysis

被引：70
作者：
Devari, Shekaraiah [1 ,2 ]
Jaglan, Sundeep [3 ]
Kumar, Manjeet [2 ]
Deshidi, Ramesh [1 ,2 ]
Guru, Santosh [4 ]
Bhushan, Shashi [4 ]
Kushwaha, Manoj [3 ]
Gupta, Ajai P. [3 ]
Gandhi, Sumit G. [4 ]
Sharma, Jai P. [3 ]
Taneja, Subhash C. [2 ]
Vishwakarma, Ram A. [2 ]
Shah, Bhahwal Ali [1 ,2 ]
机构：
[1] Acad Sci & Innovat Res, New Delhi, India
[2] CSIR Indian Inst Integrat Med, Jammu 180001, India
[3] CSIR Indian Inst Integrat Med, Qual Control & Qual Assurance Div, Jammu 180001, India
[4] CSIR Indian Inst Integrat Med, Canc Pharmacol Div, Jammu 180001, India
来源：
PHYTOCHEMISTRY | 2014年 / 98卷
关键词：
Alternaria alternata; Capsaicin; Alternariol-10-methyl ether; (MRM)-LC-ESI-MS/MS; Anti-cancer; BOSWELLIC ACIDS; PATCH; PAIN; INHIBITORS;
D O I：
10.1016/j.phytochem.2013.12.001
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Alternaria alternata, an endophytic fungus capable of producing capsaicin (1) was isolated from Capsicum annum, The endophyte was found to produce capsaicin upto three generations. Upscaling of the fermentation broth led to the isolation of one known and one compound characterized as 2,4-di-tert-butyl phenol (2) and alternario1-10-methyl ether (3) respectively. Compound 1 and 3 were identified and quantified using liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) system through multiple reaction monitoring (MRM). Furthermore, compound 3 displayed a range of cytotoxicity against a panel of human cancer cell lines and was found to induce apoptosis evidenced by Hoechst staining and loss of mitochondrial-membrane potential in HL-60 cells. (C) 2013 Elsevier Ltd. All rights reserved.
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收藏
页码：183 / 189
页数：7
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