Pregnancy and delivery rates after vitrification of in vitro-produced Nelore (Bos indicus) embryos under field conditions

被引：2
|
作者：
Pereira, Lucas C. [1 ]
Ferreira-Silva, Jose C. [1 ]
Cantanhede, Ludymila F. [1 ]
Basto, Sarah R. L. [1 ]
Veloso Neto, Humberto F. [1 ]
Moura, Marcelo T. [1 ]
Oliveira, Marcos Antonio L. [1 ]
机构：
[1] Univ Fed Rural Pernambuco, Lab Biotecn Reprod, Dept Med Vet, Av Dom Manoel de Medeiros S-N, BR-52171900 Recife, PE, Brazil
来源：
REVISTA COLOMBIANA DE CIENCIAS PECUARIAS | 2019年 / 32卷 / 01期
关键词：
cattle reproduction; cryobiology; cryoinjury; cryotolerance; embryo surplus; embryo transfer; BOVINE EMBRYOS; CRYOPRESERVATION; OOCYTES; CRYOTOLERANCE; VIABILITY;
D O I：
10.17533/udea.rccp.v32n1a05
中图分类号：
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号：
0905 ;
摘要：
Background: Cryopreservation preserves cellular viability under low temperatures, resulting in diminished intracellular enzymatic activity and reduced cellular metabolism that ultimately allows preserving genetic material for indefinite periods of time. Embryos submitted to cryopreservation suffer from considerable morphological and functional damage, resulting in reduced survival and development rates.Objective: To evaluate pregnancy and delivery rates of in vitro-produced (IVP) Nellore (Bos indicus) embryos after vitrification under field conditions. Methods: The IVP embryos at blastocyst (Bl) and expanded blastocyst (Bx) were transferred fresh (n= 137) or after vitrification (n= 127).Results: Pregnancy rates at 35 d for fresh embryos were lower in Bl (41.6) than in Bx (60.9) (p<0.05). After vitrification, pregnancy rates were similar at 35 d between Bl (38.0) and Bx (47.6) (p>0.05). Pregnancy loss at 60 d were similar (p>0.05) for both fresh (Bl: 3.1 and Bx: 4.8) and vitrified embryos (Bl: 1.9 and Bx: 4.7). Delivery rates were similar between groups (p>0.05). Conclusion: Both pregnancy and delivery rates of Bos indicus IVP embryos vitrified under field conditions are indistinguishable from fresh embryos.
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页码：43 / 49
页数：7
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