Putting Molecules in Their Place

被引：24

作者：

Cinquin, Bertrand P. ^{[1
]}

Do, Myan ^{[1
]}

McDermott, Gerry ^{[1
]}

Walters, Alison D. ^{[2
]}

Myllys, Markko ^{[3
]}

Smith, Elizabeth A. ^{[1
]}

Cohen-Fix, Orna ^{[2
]}

Le Gros, Mark A. ^{[1
,4
]}

Larabell, Carolyn A. ^{[1
,4
]}

机构：

[1] Univ Calif San Francisco, Dept Anat, San Francisco, CA 94143 USA

[2] NIDDK, NIH, Bethesda, MD 20892 USA

[3] Univ Jyvaskyla, Dept Phys, Jyvaskyla, Finland

[4] Univ Calif Berkeley, Lawrence Berkeley Natl Lab, Phys Biosci Div, Berkeley, CA 94720 USA

来源：

JOURNAL OF CELLULAR BIOCHEMISTRY | 2014年 / 115卷 / 02期

关键词：

CORRELATED; IMAGING; FLUORESCENCE; MICROSCOPY; SOFT X-RAY; TOMOGRAPHY; X-RAY TOMOGRAPHY; ELECTRON-MICROSCOPY; CORRELATED LIGHT; CRYOELECTRON TOMOGRAPHY; FLUORESCENCE; PROTEINS; SUPERRESOLUTION; ARCHITECTURE; SPECIMENS; LOCATION;

D O I：

10.1002/jcb.24658

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Each class of microscope is limited to imaging specific aspects of cell structure and/or molecular organization. However, imaging the specimen by complementary microscopes and correlating the data can overcome this limitation. Whilst not a new approach, the field of correlative imaging is currently benefitting from the emergence of new microscope techniques. Here we describe the correlation of cryogenic fluorescence tomography (CFT) with soft X-ray tomography (SXT). This amalgamation of techniques integrates 3D molecular localization data (CFT) with a high-resolution, 3D cell reconstruction of the cell (SXT). Cells are imaged in both modalities in a near-native, cryopreserved state. Here we describe the current state of the art in correlative CFT-SXT, and discuss the future outlook for this method. J. Cell. Biochem. 115: 209-216, 2014. (c) 2013 Wiley Periodicals, Inc.

引用

页码：209 / 216

页数：8

共 55 条

[1]

Alberts B., 2008, Molecular biology of the cell (Garland Science Pub), V5th, P1392

[2]

[Anonymous], 2001, Mathematical methods in image reconstruction

[3]

Attwood D., 1999, SOFT XRAYS EXTREME U, P470

[4] Electron tomography of molecules and cells [J].

Baumeister, W ;

Grimm, R ;

Walz, J .

TRENDS IN CELL BIOLOGY, 1999, 9 (02) :81-85

[5] Imaging cellular structure across scales with correlated light, superresolution, and electron microscopy [J].

Briggs, John A. G. ;

Lakadamyali, Melike .

MOLECULAR BIOLOGY OF THE CELL, 2012, 23 (06) :979-980

[6] The power of correlative microscopy: multi-modal, multi-scale, multi-dimensional [J].

Caplan, Jeffrey ;

Niethammer, Marc ;

Taylor, Russell M., II ;

Czymmek, Kirk J. .

CURRENT OPINION IN STRUCTURAL BIOLOGY, 2011, 21 (05) :686-693

[7] Demonstration of 12 nm Resolution Fresnel Zone Plate Lens based Soft X-ray Microscopy [J].

Chao, Weilun ;

Kim, Jihoon ;

Rekawa, Senajith ;

Fischer, Peter ;

Anderson, Erik H. .

OPTICS EXPRESS, 2009, 17 (20) :17669-17677

[8] Nuclear Aggregation of Olfactory Receptor Genes Governs Their Monogenic Expression [J].

Clowney, E. Josephine ;

LeGros, Mark A. ;

Mosley, Colleen P. ;

Clowney, Fiona G. ;

Markenskoff-Papadimitriou, Eirene C. ;

Myllys, Markko ;

Barnea, Gilad ;

Larabell, Carolyn A. ;

Lomvardas, Stavros .

CELL, 2012, 151 (04) :724-737

[9] Fluorescent Proteins: Shine on, You Crazy Diamond [J].

Dedecker, Peter ;

De Schryver, Frans C. ;

Hofkens, Johan .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2013, 135 (07) :2387-2402

[10] 3-DIMENSIONAL STRUCTURE OF LIPID VESICLES EMBEDDED IN VITREOUS ICE AND INVESTIGATED BY AUTOMATED ELECTRON TOMOGRAPHY [J].

DIERKSEN, K ;

TYPKE, D ;

HEGERL, R ;

WALZ, J ;

SACKMANN, E ;

BAUMEISTER, W .

BIOPHYSICAL JOURNAL, 1995, 68 (04) :1416-1422

← 1 2 3 4 5 6 →