Developmental validation of a real-time PCR assay for the simultaneous quantification of total human and male DNA

被引：50

作者：

Krenke, Benjamin E. ^{[1
]}

Nassif, Nadine ^{[1
]}

Sprecher, Cynthia J. ^{[1
]}

Knox, Curtis ^{[1
]}

Schwandt, Melissa ^{[1
]}

Storts, Douglas R. ^{[1
]}

机构：

[1] Promega Corp, Madison, WI 53711 USA

来源：

FORENSIC SCIENCE INTERNATIONAL-GENETICS | 2008年 / 3卷 / 01期

关键词：

Forensic science; DNA quantification; Real-time PCR; QPCR;

D O I：

10.1016/j.fsigen.2008.07.004

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Multiplex human short tandem repeat analysis demands reliable DNA quantification to consistently produce interpretable genotypes. The Plexor (R) HY System is a multiplex quantitative PCR assay to quantify total human and male DNA. We performed developmental validation of the Plexor (R) HY System to demonstrate the performance capabilities and limitations of the assay for forensic applications. Validation studies examined: (a) human specificity, (b) sensitivity, (c) quantification of degraded DNA, (d) impact of inhibitors, (e) male/female mixture and Y-assay male specificity, (0 reproducibility and concordance and (g) population studies. (c) 2008 Elsevier Ireland Ltd. All rights reserved.

引用

页码：14 / 21

页数：8

共 23 条

[1]

[Anonymous], PROMEGA NOTES

[2] Transcription of the human U2 snRNA genes continues beyond the 3′ box in vivo [J].