A two-photon fluorescent probe for imaging hydrogen sulfide in living cells

被引：88
|
作者：
Liu, Tianyu [1 ,2 ]
Zhang, Xinfu [1 ]
Qiao, Qinglong [1 ]
Zou, Chunyan [1 ]
Feng, Lei [1 ]
Cui, Jingnan [1 ]
Xu, Zhaochao [1 ,2 ]
机构：
[1] Dalian Univ Technol, State Key Lab Fine Chem, Dalian 116012, Peoples R China
[2] Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
来源：
DYES AND PIGMENTS | 2013年 / 99卷 / 03期
关键词：
Two-photon fluorescence microscopy; Fluorescent probes; Hydrogen sulfide; Thiolysis; High selectivity; Cells imaging; CHEMOSENSOR; CHEMODOSIMETER; EMISSION; H2S;
D O I：
10.1016/j.dyepig.2013.06.031
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
Fluorescent probes for hydrogen sulfide have received considerable attention because of the biological significance of H2S recognized recently. Two-photo microscopy offers advantages of increased penetration depth, localized excitation, and prolonged observation time. However, two-photon fluorescent probes for H2S are still rare. In this work, we introduced a dinitrophenyl ether group into the 4-position of 1,8-naphthalimide, which acts as the H2S reactive site, to efficiently yield compound NI-NHS as a two-photo fluorescent probe for H2S. The probe NI-NHS has a high selectivity for H2S over competitive anions and sulfide-containing analytes. This probe exhibits turn-on fluorescence detection of H2S in bovine serum and two-photon fluorescent imaging of H2S in living cells. (C) 2013 Elsevier Ltd. All rights reserved.
引用
收藏
页码：537 / 542
页数：6
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