The Voltage-Gated Sodium Channel Nav1.8 Is Expressed in Human Sperm

被引：28

作者：

Cejudo-Roman, Antonio ^{[1
]}

Pinto, Francisco M. ^{[1
]}

Subiran, Nerea ^{[2
]}

Ravina, Cristina G. ^{[3
]}

Fernandez-Sanchez, Manuel ^{[3
]}

Perez-Hernandez, Natalia ^{[1
]}

Perez, Ricardo ^{[1
]}

Pacheco, Alberto ^{[4
]}

Irazusta, Jon ^{[2
]}

Candenas, Luz ^{[1
]}

机构：

[1] Univ Seville, CSIC, IIQ, Seville, Spain

[2] Univ Basque Country, Fac Med & Odontol, Dept Fisiol, Leioa, Bizkaia, Spain

[3] Inst Valenciano Infertilidad, Seville, Spain

[4] Inst Madrileno Infertilidad, Madrid, Spain

来源：

PLOS ONE | 2013年 / 8卷 / 09期

关键词：

HUMAN SPERMATOZOA; CATSPER CHANNEL; MALE-FERTILITY; CA2+ CHANNEL; CAPACITATION; DIVERSITY; CALCIUM; TACHYKININS; ACTIVATION; PRINCIPAL;

D O I：

10.1371/journal.pone.0076084

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The role of Na+ fluxes through voltage-gated sodium channels in the regulation of sperm cell function remains poorly understood. Previously, we reported that several genes encoding voltage-gated Na+ channels were expressed in human testis and mature spermatozoa. In this study, we analyzed the presence and function of the TTX-resistant VGSC a subunit Na(v)1.8 in human capacitated sperm cells. Using an RT-PCR assay, we found that the mRNA of the gene SCN10A, that encode Na-v1.8, was abundantly and specifically expressed in human testis and ejaculated spermatozoa. The Na-v1.8 protein was detected in capacitated sperm cells using three different specific antibodies against this channel. Positive immunoreactivity was mainly located in the neck and the principal piece of the flagellum. The presence of Na-v1.8 in sperm cells was confirmed by Western blot. Functional studies demonstrated that the increases in progressive motility produced by veratridine, a voltage-gated sodium channel activator, were reduced in sperm cells preincubated with TTX (10 mu M), the Na-v1.8 antagonist A-803467, or a specific Na-v1.8 antibody. Veratridine elicited similar percentage increases in progressive motility in sperm cells maintained in Ca2+-containing or Ca2+-free solution and did not induce hyperactivation or the acrosome reaction. Veratridine caused a rise in sperm intracellular Na+, [Na+](i), and the sustained phase of the response was inhibited in the presence of A-803467. These results verify that the Na+ channel Na-v1.8 is present in human sperm cells and demonstrate that this channel participates in the regulation of sperm function.

引用

页数：13

共 44 条

[1] The tetrodotoxin-resistant sodium channel SNS has a specialized function in pain pathways [J].