Prion protein (PrPc) interacts with histone H3 confirmed by affinity chromatography

被引：3

作者：

Cai, Hanning ^{[1
]}

Xie, Ying ^{[1
]}

Hu, Lingyin ^{[1
]}

Fan, Jingjing ^{[1
]}

Li, Renqiang ^{[1
]}

机构：

[1] Jinan Univ, Dept Biotechnol, Guangzhou 510632, Guangdong, Peoples R China

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2013年 / 929卷

关键词：

Prion protein; Histone H3; Interaction; Affinity chromatography; CHROMATIN; EXPRESSION;

D O I：

10.1016/j.jchromb.2013.04.003

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The histones including H2a, H2b, H3 and H4 purified from pig liver tissue were immobilized onto Sepharose 4B to create a histone-Sepharose column. During chromatography of cow milk casein by histone-Sepharose column, two isoforms of prion protein (PrPc) with 34 and 30 kDa molecular mass corresponding to diglycosylated and monoglycosylated PrPc respectively were found to be captured by histone ligands. To further verify the interaction between histones and PrPc, the PrPc-Sepharose column was prepared and used to separate the histones. Two chromatography processes and SOS-PAGE demonstrated that only H3 in the histones was found to interact with PrPc. This study suggested H3 could be the target molecule of PrPc in nuclei, which might be useful for understanding the prion disease. (C) 2013 Elsevier B.V. All rights reserved.

引用

页码：40 / 44

页数：5

共 18 条

[1] Prion and prejudice: normal protein and the synapse
Brown, DR
[J]. TRENDS IN NEUROSCIENCES, 2001, 24 (02) : 85 - 90
[2] DILRINGER C, 2003, J BIOL CHEM, V278, P42131
[3] Selective expression of prion protein in peripheral tissues of the adult mouse
Ford, MJ
Burton, LJ
Morris, RJ
Hall, SM
[J]. NEUROSCIENCE, 2002, 113 (01) : 177 - 192
[4] Prion Protein in Milk
Franscini, Nicola
El Gedaily, Ahmed
Matthey, Ulrich
Franitza, Susanne
Sy, Man-Sun
Buerkle, Alexander
Groschup, Martin
Braun, Ueli
Zahn, Ralph
[J]. PLOS ONE, 2006, 1 (01):
[5] Histone acetylation in chromatin structure and transcription
Grunstein, M
[J]. NATURE, 1997, 389 (6649) : 349 - 352
[6] Eukaryotic transcription: An interlaced network of transcription factors and chromatin-modifying machines
Kadonaga, JT
[J]. CELL, 1998, 92 (03) : 307 - 313
[7] Twenty-five years of the nucleosome, fundamental particle of the eukaryote chromosome
Kornberg, RD
Lorch, YL
[J]. CELL, 1999, 98 (03) : 285 - 294
[8] CHROMATIN AND GENE-EXPRESSION - CONSTANT QUESTIONS, BUT CHANGING ANSWERS
LEWIN, B
[J]. CELL, 1994, 79 (03) : 397 - 406
[9] The diverse functions of histone lysine methylation
Martin, C
Zhang, Y
[J]. NATURE REVIEWS MOLECULAR CELL BIOLOGY, 2005, 6 (11) : 838 - 849
[10] New insights into cellular prion protein (PrPc) functions: The "ying and yang" of a relevant protein
Nicolas, Oriol
Gavin, Rosalina
del Rio, Jose A.
[J]. BRAIN RESEARCH REVIEWS, 2009, 61 (02) : 170 - 184

← 1 2 →