A fast responsive, highly selective and light-up fluorescent probe for the two-photon imaging of carboxylesterase in living cells

被引：40

作者：

Wang, Jianguo ^{[1
]}

Chen, Qingqing ^{[1
]}

Tian, Na ^{[2
]}

Zhu, Wenping ^{[1
]}

Zou, Hang ^{[3
,4
]}

Wang, Xuesong ^{[2
]}

Li, Xiaokang ^{[1
]}

Fan, Xiaolin ^{[1
]}

Jiang, Guoyu ^{[1
]}

Tang, Ben Zhong ^{[3
,4
]}

机构：

[1] Gannan Normal Univ, Key Lab Organopharmaceut Chem, Ganzhou 341000, Peoples R China

[2] Chinese Acad Sci, Tech Inst Phys & Chem, Key Lab Photochem Convers & Optoelect Mat, Beijing 100190, Peoples R China

[3] Hong Kong Univ Sci & Technol, Dept Chem, Inst Adv Study, Inst Mol Funct Mat, Kowloon, Hong Kong, Peoples R China

[4] Hong Kong Univ Sci & Technol, State Key Lab Mol Neurosci, Kowloon, Hong Kong, Peoples R China

来源：

JOURNAL OF MATERIALS CHEMISTRY B | 2018年 / 6卷 / 11期

基金：

中国国家自然科学基金;

关键词：

OFF-ON PROBE; MASS-SPECTROMETRY; NITROXYL HNO; ESTERASE; TISSUES; PRODRUGS; ACTIVATION; MICROSCOPY; DESIGN; ACID;

D O I：

10.1039/c8tb00147b

中图分类号：

TB3 [工程材料学]; R318.08 [生物材料学];

学科分类号：

0805 ; 080501 ; 080502 ;

摘要：

A fast responsive and two photon fluorescent probe (HCyNAc) for carboxylesterase (CaE) has been designed based on the D--A structure of hemicyanine and naphthalene derivatives. After enzymatic reaction, HCyNAc enabled light-up fluorescence assay of CaE over other biologically-relevant species and enzymes, including ROS. Two-photon imaging of endogenous CaE was confirmed in HeLa cells using HCyNAc under 800 nm NIR excitation.

引用

页码：1595 / 1599

页数：5

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