Molecular investigation of carbapenem resistance among multidrug-resistant Pseudomonas aeruginosa isolated clinically in Thailand

被引：22
作者：
Khuntayaporn, Piyatip [1 ]
Montakantikul, Preecha [2 ]
Santanirand, Pitak [3 ]
Kiratisin, Pattarachai [4 ]
Chomnawang, Mullika Traidej [1 ]
机构：
[1] Mahidol Univ, Dept Microbiol, Rachathevi Bangkok 10400, Thailand
[2] Mahidol Univ, Dept Pharm, Fac Pharm, Rachathevi Bangkok 10400, Thailand
[3] Mahidol Univ, Fac Med, Ramathibodi Hosp, Dept Pathol, Toong Phayathai 10400, Rachathevi Bang, Thailand
[4] Mahidol Univ, Siriraj Hosp, Fac Med, Dept Microbiol, Bangkok 10700, Thailand
来源：
MICROBIOLOGY AND IMMUNOLOGY | 2013年 / 57卷 / 03期
关键词：
-lactamases; carbapenem resistance mechanisms; efflux pump systems; multidrug-resistant Pseudomonas aeruginosa; METALLO-BETA-LACTAMASES; ANTIMICROBIAL RESISTANCE; OUTER-MEMBRANE; ACTIVE EFFLUX; MECHANISMS; EXPRESSION; AMPC; OPRD; HYPERSUSCEPTIBILITY; ANTIBIOTICS;
D O I：
10.1111/1348-0421.12021
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
Carbapenem resistant Pseudomonas aeruginosa were isolated among multidrug-resistant (CR-MDR) organisms from tertiary hospitals in Thailand. Decreased expression of oprD mRNA (93.65%) was predominant followed by increased expression of mexAB-oprM mRNA (92.06%) and mexXY mRNA (63.49%). Interestingly, 23 of 126 (18.25%) isolates were susceptible to imipenem with down-regulated oprD expression and non-up-regulated mexCD-oprJ mRNA expression. Metallo--lactamases production was clearly positive in 24 isolates (18.46%) and weakly positive in 12 isolates (9.23%). Among both of these sets of isolates, imp-1, imp-14 and vim-2 were identified. Hyperproduction of AmpC -lactamase had the lowest prevalence rate (3.97%). It was concluded that CR-MDR P. aeruginosa clinical isolates in Thailand possess multifactorial resistance mechanisms.
引用
收藏
页码：170 / 178
页数：9
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