A compact, multidimensional spectrofluorometer exploiting supercontinuum generation

被引：34

作者：

Manning, Hugh B. ^{[1
]}

Kennedy, Gordon T. ^{[2
]}

Owen, Dylan M. ^{[1
]}

Grant, David M. ^{[1
]}

Magee, Anthony I. ^{[3
]}

Neil, Mark A. A. ^{[2
]}

Itoh, Yoshifumi ^{[4
]}

Dunsby, Chris ^{[2
]}

French, Paul M. W. ^{[2
]}

机构：

[1] Univ London Imperial Coll Sci Technol & Med, Dept Chem, Chem Biol Ctr, London SW7 2AY, England

[2] Univ London Imperial Coll Sci Technol & Med, Dept Phys, Photon Grp, London SW7 2AZ, England

[3] Univ London Imperial Coll Sci Technol & Med, Natl Heart & Lung Inst, London, England

[4] Univ London Imperial Coll Sci Technol & Med, Kennedy Inst Rheumatol, Dept Matrix Biol, London SW7 2AY, England

来源：

JOURNAL OF BIOPHOTONICS | 2008年 / 1卷 / 06期

基金：

英国医学研究理事会; 英国工程与自然科学研究理事会; 英国生物技术与生命科学研究理事会;

关键词：

fluorescence; fluorescence lifetime; anisotropy; time-resolved anisotropy; spectrofluorometer; supercontinuum; di-4-ANEPPDHQ; FRET;

D O I：

10.1002/jbio.200810051

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We report a novel, compact and automated multidimensioual spectrofluorometer that exploits a fibre-laser-pumped ultrafast supercontinuum source to provide resolution with respect to intensity, excitation and emission wavelength, decay time and polarisation. This instrument has been applied to study the photophysics of the phase-sensitive membrane probe di-4-ANEPPDHQ and to characterise protein-protein interactions via Forster resonance energy transfer. It can be applied to in situ measurements via a fibre-optic probe in medical and other contexts and is demonstrated here to provide a comprehensive characterisation of tissue autofluorescence. [GRAPHICS] A photograph of the multidimensional spectrofluorometer. (c) 2008 by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim

引用

页码：494 / 505

页数：12

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