Detection and characterization of Shiga toxin-producing Escherichia coli in faeces and lymphatic tissue of free-ranging deer

被引：18

作者：

Eggert, M. ^{[1
]}

Stueber, E. ^{[2
]}

Heurich, M. ^{[3
]}

Fredriksson-Ahomaa, M. ^{[4
]}

Burgos, Y. ^{[5
]}

Beutin, L. ^{[5
]}

Maertlbauer, E. ^{[2
]}

机构：

[1] Univ Munich, Inst Human Genet, Univ Hosp, D-85764 Oberschleissheim, Germany

[2] Univ Munich, Inst Food Sci, Dept Vet Sci, Fac Vet Med, D-85764 Oberschleissheim, Germany

[3] Bavarian Forest Natl Pk, Dept Res & Documentat, Grafenau, Germany

[4] Univ Helsinki, Dept Food Hyg & Environm Hlth, Fac Vet Med, FIN-00014 Helsinki, Finland

[5] BfR Fed Inst Risk Assessment, Natl Reference Lab Escherichia Coli, Berlin, Germany

来源：

EPIDEMIOLOGY AND INFECTION | 2013年 / 141卷 / 02期

关键词：

Bacterial typing; Shiga toxin-producing E. coli; SALMONELLA SPP; CAMPYLOBACTER SPP; STRAINS; O157H7; O157-H7; GENE; PCR; IDENTIFICATION; SPECIFICITY; INFECTIONS;

D O I：

10.1017/S0950268812000246

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

Shiga toxin-producing Escherichia coli (STEC) have led to outbreaks worldwide and are considered emerging pathogens. Infections by STEC in humans have been reported after consumption of mainly beef, but also deer. This study investigated the occurrence of STEC in deer in Germany. The virulence genes eae, e-hlyA and saa, the stx subtypes, pulsed-field gel electrophoresis (PFGE) patterns and serovars were studied. In total, 120 samples of 60 animals were screened by real-time polymerase chain reaction (PCR). The PCR results showed a high detection rate of stx genes (83%). Mainly faecal samples, but also some lymphatic tissue samples, tested stx-positive. All isolates carried stx2, were eae-negative and carried e-hlyA in 38% and saa in 9% of samples. Serovars (O88:[H8], O174:[H8], O146:H28) associated with human diseases were also identified. In some animals, isolates from lymphatic tissue and faecal samples showed undistinguishable PFGE patterns. The examined deer were shown to be relevant reservoirs of STEC with subtype stx2b predominating.

引用

页码：251 / 259

页数：9

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