cAMPr: A single-wavelength fluorescent sensor for cyclic AMP

被引：42

作者：

Hackley, Christopher R. ^{[1
]}

Mazzoni, Esteban O. ^{[1
,2
]}

Blau, Justin ^{[1
,2
,3
]}

机构：

[1] NYU, Dept Biol, 100 Washington Sq East, New York, NY 10003 USA

[2] NYU, Langone Med Ctr, Neurosci Inst, New York, NY 10016 USA

[3] NYU Abu Dhabi Inst, Ctr Genom & Syst Biol, Abu Dhabi, U Arab Emirates

来源：

SCIENCE SIGNALING | 2018年 / 11卷 / 520期

关键词：

CIRCADIAN-RHYTHMS; EMBRYONIC STEM; CRYSTAL-STRUCTURE; NEURONAL-ACTIVITY; DROSOPHILA BRAIN; CLOCK NEURONS; RI-ALPHA; PROTEIN; PDF; PKA;

D O I：

10.1126/scisignal.aah3738

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Genetically encoded fluorescent sensors enable cell-specific measurements of ions and small molecules in real time. Cyclic adenosine monophosphate (cAMP) is one of the most important signaling molecules in virtually all cell types and organisms. We describe cAMPr, a new single-wavelength cAMP sensor. We developed cAMPr in bacteria and embryonic stem cells and validated the sensor in mammalian neurons in vitro and in Drosophila circadian pacemaker neurons in intact brains. Comparison with other single-wavelength cAMP sensors showed that cAMPr improved the quantitative detection of cAMP abundance. In addition, cAMPr is compatible with both single-photon and two-photon imaging. This enabled us to use cAMPr together with the red fluorescent Ca2+ sensor RCaMP1h to simultaneously monitor Ca2+ and cAMP in Drosophila brains. Thus, cAMPr is a new and versatile genetically encoded cAMP sensor.

引用

页数：11