A Transgenic MMTV-Flippase Mouse Line for Molecular Engineering in Mammary Gland and Breast Cancer Mouse Models

被引：6

作者：

Luond, Fabiana ^{[1
]}

Bill, Ruben ^{[1
]}

Vettiger, Andrea ^{[1
,2
]}

Oller, Heide ^{[3
]}

Pelczar, Pawel ^{[3
]}

Christofori, Gerhard ^{[1
]}

机构：

[1] Univ Basel, Dept Biomed, Mattenstr 28, CH-4058 Basel, Switzerland

[2] Biozentrum Univ Basel, Focal Area Infect Biol, Klingelbergstr 50-70, CH-4056 Basel, Switzerland

[3] Univ Basel, Ctr Transgen Models, Mattenstr 22, CH-4058 Basel, Switzerland

来源：

JOURNAL OF MAMMARY GLAND BIOLOGY AND NEOPLASIA | 2019年 / 24卷 / 01期

基金：

瑞士国家科学基金会;

关键词：

Breast cancer; Flippase; Lineage tracing; Mammary gland; MMTV promoter; Transgenic mice; SITE-SPECIFIC RECOMBINASES; MULTIPOTENCY; METASTASIS; PLASTICITY; CELLS;

D O I：

10.1007/s10911-018-9412-4

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Genetically engineered mouse models have become an indispensable tool for breast cancer research. Combination of multiple site-specific recombination systems such as Cre/loxP and Flippase (Flp)/Frt allows for engineering of sophisticated, multi-layered conditional mouse models. Here, we report the generation and characterization of a novel transgenic mouse line expressing a mouse codon-optimized Flp under the control of the mouse mammary tumor virus (MMTV) promoter. These mice show robust Flp-mediated recombination in luminal mammary gland and breast cancer cells but no Flp activity in non-mammary tissues, with the exception of limited activity in salivary glands. These mice provide a unique tool for studying mammary gland biology and carcinogenesis in mice.

引用

页码：39 / 45

页数：7

共 20 条

[1] Projections and interconnections of genetically defined serotonin neurons in mice [J].

Bang, Sun Jung ;

Jensen, Patricia ;

Dymecki, Susan M. ;

Commons, Kathryn G. .

EUROPEAN JOURNAL OF NEUROSCIENCE, 2012, 35 (01) :85-96

[2] Plasticity between Epithelial and Mesenchymal States Unlinks EMT from Metastasis-Enhancing Stem Cell Capacity [J].

Beerling, Evelyne ;

Seinstra, Danielle ;

de Wit, Elzo ;

Kester, Lennart ;

van der Velden, Daphne ;

Maynard, Carrie ;

Schafer, Ronny ;

van Diest, Paul ;

Voest, Emile ;

van Oudenaarden, Alexander ;

Vrisekoop, Nienke ;

van Rheenen, Jacco .

CELL REPORTS, 2016, 14 (10) :2281-2288

[3] Lgr6 labels a rare population of mammary gland progenitor cells that are able to originate luminal mammary tumours [J].

Blaas, Leander ;

Pucci, Fabio ;

Messal, Hendrik A. ;

Andersson, Agneta B. ;

Ruiz, E. Josue ;

Gerling, Marco ;

Douagi, Iyadh ;

Spencer-Dene, Bradley ;

Musch, Alexandra ;

Mitter, Richard ;

Bhaw, Leena ;

Stone, Richard ;

Bornhorst, Dorothee ;

Sesay, Abdul K. ;

Jonkers, Jos ;

Stamp, Gordon ;

Malanchi, Ilaria ;

Toftgard, Rune ;

Behrens, Axel .

NATURE CELL BIOLOGY, 2016, 18 (12) :1346-+

[4] Choosing a Mouse Model: Experimental Biology in Context - The Utility and Limitations of Mouse Models of Breast Cancer [J].

Borowsky, Alexander D. .

COLD SPRING HARBOR PERSPECTIVES IN BIOLOGY, 2011, 3 (09) :1-16

[5] Talking about a revolution: The impact of site-specific recombinases on genetic analyses in mice [J].

Branda, CS ;

Dymecki, SM .

DEVELOPMENTAL CELL, 2004, 6 (01) :7-28

[6] Polyclonal breast cancer metastases arise from collective dissemination of keratin 14-expressing tumor cell clusters [J].

Cheung, Kevin J. ;

Padmanaban, Veena ;

Silvestri, Vanesa ;

Schipper, Koen ;

Cohen, Joshua D. ;

Fairchild, Amanda N. ;

Gorin, Michael A. ;

Verdone, James E. ;

Pienta, Kenneth J. ;

Bader, Joel S. ;

Ewald, Andrew J. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2016, 113 (07) :E854-E863

[7] MAPPING CELL FATE AND FUNCTION USING RECOMBINASE-BASED INTERSECTIONAL STRATEGIES [J].

Dymecki, Susan M. ;

Ray, Russell S. ;

Kim, Jun C. .

METHODS IN ENZYMOLOGY, VOL 477: GUIDE TO TECHNIQUES IN MOUSE DEVELOPMENT, PART B: MOUSE MOLECULAR GENETICS, SECOND EDITION, 2010, 477 :183-213

[8] Islet1 Derivatives in the Heart Are of Both Neural Crest and Second Heart Field Origin [J].

Engleka, Kurt A. ;

Manderfield, Lauren J. ;

Brust, Rachael D. ;

Li, Li ;

Cohen, Ashley ;

Dymecki, Susan M. ;

Epstein, Jonathan A. .

CIRCULATION RESEARCH, 2012, 110 (07) :922-U47

[9] Epithelial-to-mesenchymal transition is not required for lung metastasis but contributes to chemoresistance [J].

Fischer, Kari R. ;

Durrans, Anna ;

Lee, Sharrell ;

Sheng, Jianting ;

Li, Fuhai ;

Wong, Stephen T. C. ;

Choi, Hyejin ;

El Rayes, Tina ;

Ryu, Seongho ;

Troeger, Juliane ;

Schwabe, Robert F. ;

Vahdat, Linda T. ;

Altorki, Nasser K. ;

Mittal, Vivek ;

Gao, Dingcheng .

NATURE, 2015, 527 (7579) :472-+

[10] INDUCTION OF MAMMARY-TUMORS BY EXPRESSION OF POLYOMAVIRUS MIDDLE T-ONCOGENE - A TRANSGENIC MOUSE MODEL FOR METASTATIC DISEASE [J].

GUY, CT ;

CARDIFF, RD ;

MULLER, WJ .

MOLECULAR AND CELLULAR BIOLOGY, 1992, 12 (03) :954-961

← 1 2 →