Elevated expression of EP4 in human decidua is associated with delayed embryo expulsion during medical abortion by promoting decidual cell proliferation

被引：2

作者：

Feng, Wenhua ^{[1
]}

Ma, Chao ^{[1
]}

Gao, Guoqiang ^{[2
]}

Wang, Hong ^{[1
]}

Sui, Yang ^{[1
]}

Xu, Wei ^{[1
]}

Liu, Wei ^{[1
]}

Han, Weitian ^{[1
]}

Li, Hongtu ^{[1
]}

机构：

[1] China Med Univ, Liaoning Prov Res Inst Family Planning, Natl Hlth & Family Planning Commiss, Key Lab Reprod Hlth & Med Genet, 10 PuHe St, Shenyang, Liaoning, Peoples R China

[2] Tengzhou Cent Peoples Hosp, Tengzhou, Peoples R China

来源：

JOURNAL OF MATERNAL-FETAL & NEONATAL MEDICINE | 2021年 / 34卷 / 01期

关键词：

Deicidua; EP4; medical abortion; prostanoid receptor; PROSTANOID RECEPTORS; INTERNATIONAL UNION; PREGNANCY; PROSTAGLANDIN-E2; CLASSIFICATION; LOCALIZATION; MIFEPRISTONE;

D O I：

10.1080/14767058.2019.1587405

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

Purpose: Mifepristone in conjunction with misoprostol, is widely used in China as an effective medical abortifacient. However, a small proportion of women experience the unpleasant side effects of prolonged vaginal bleeding caused by delayed embryo expulsion. The aims of this study were to determine whether the expression levels of prostanoid receptors in human decidua are associated with delayed embryo expulsion in mifepristone-misoprostol induced an early medical abortion. Methods: Discharged decidua tissues were collected from females undergoing an artificial abortion (AA) (n = 28), females with early embryo expulsion during a medical abortion (EEMA) (n = 20) and delayed embryo expulsion in medical abortion (DEMA) (n = 30). The expression levels of prostanoid receptors in human decidua were assessed with immunohistochemistry and real-time PCR methods. Further, the RNAi method was used to silence prostanoid receptors 4 (EP4) in the primary decidual cells and human endometrial adenocarcinoma cell line Ishikawa cells in vitro and cell cycle analysis of these cells was performed. Results: All five prostanoid receptors (EP1-4, FP) were observed in human early pregnancy decidua. The protein and mRNA expression level of EP4 in the DEMA group were all significantly higher than that in the EEMA group. EP4 silence induced G1/S arrest of primary decidual cells and Ishikawa cells in vitro. Conclusions: Elevated expression level of EP4 in human decidua was significantly associated with delayed embryo expulsion in early medical abortion by promoting decidual cell proliferation. Detailed studies on the nature of roles EP4 plays in human decidua will help us to develop more effective prevention and noninvasive intervention approaches for delayed embryo expulsion during a medical abortion.

引用

页码：41 / 48

页数：8

共 23 条

[1] Selective Inhibition of Prostaglandin E2 Receptors EP2 and EP4 Induces Apoptosis of Human Endometriotic Cells through Suppression of ERK1/2, AKT, NFκB, and β-Catenin Pathways and Activation of Intrinsic Apoptotic Mechanisms [J].