OEM-A new medium for rapid isolation of onion-pathogenic and onion-associated bacteria

被引：20

作者：

Zaid, Ali M. ^{[1
]}

Bonasera, Jean M. ^{[1
]}

Beer, Steven V. ^{[1
]}

机构：

[1] Cornell Univ, Dept Plant Pathol & Plant Microbe Biol, Ithaca, NY 14853 USA

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2012年 / 91卷 / 03期

关键词：

Onion extract; Semi-selective medium; Onion-pathogenic bacteria; PANTOEA-ANANATIS; 1ST REPORT; ENTEROBACTER-CLOACAE; CAUSAL AGENT; SELECTIVE ISOLATION; LEAF-BLIGHT; BULB DECAY; CENTER ROT; DISEASE; ENUMERATION;

D O I：

10.1016/j.mimet.2012.09.031

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Onions (Allium cepa L) are plagued by a number of bacterial pathogens including Pantoea ananatis, P. agglomerans, Burkholderia cepacia, Enterobacter cloacae, Pectobacterium carotovorum subsp. carotovorum, Xanthomonas axonopodis pv. axonopodis and several Pseudomonas spp. We developed a semi-selective medium, termed onion extract medium (OEM), to selectively and rapidly isolate bacteria pathogenic to and associated with onions and onion-related samples including bulbs, seeds, sets, transplant seedlings, soil and water. Most strains of interest grow sufficiently on OEM in 24 h at 28 degrees C for tentative identification based on colony morphology, facilitating further characterization by microbiological and/or molecular means. (c) 2012 Elsevier B.V. All rights reserved.

引用

页码：520 / 526

页数：7

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