Identification of group I porcine enteroviruses by monoclonal antibodies in cell culture

被引：12

作者：

Dauber, M ^{[1
]}

机构：

[1] Fed Res Ctr Virus Dis Anim, Friedrich Loeffler Inst, Inst Diagnost Virol, D-17498 Insel Riems, Germany

来源：

VETERINARY MICROBIOLOGY | 1999年 / 67卷 / 01期

关键词：

pig-viruses; porcine enterovirus; monoclonal antibody; diagnosis; immunofluorescence;

D O I：

10.1016/S0378-1135(99)00024-3

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A panel of monoclonal antibodies (mAb) against porcine enteroviruses (PEV) was established. One of these mAbs reacts group-specifically with PEV of serotype group I in the indirect immunofluorescence assay (IIF). This mAb is very well suited for diagnosis of PEV infections. However, the mAb neither neutralizes virus nor does it react with virus particles in immune electron microscopy (IEM). Another mAb is PEV-1 specific in IIF, neutralizes virus, and is suited for IEM. Both mAbs presumably recognize conformation-dependent epitopes of the virus. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：1 / 12

页数：12

共 32 条

[1]

ALEXANDER TJ, 1967, RES VET SCI, V8, P330

[2]

APPEL G, 1995, TIERAERZTL UMSCHAU, V50, P326

[3] AN ENZYME-LINKED IMMUNOSORBENT-ASSAY (ELISA) FOR THE DETECTION AND QUANTIFICATION OF ANTIBODIES AGAINST SWINE VESICULAR DISEASE VIRUS (SVDV) [J].