Imbalance in superoxide dismutase/thioredoxin reductase activities in hypercholesterolemic subjects: relationship with low density lipoprotein oxidation

被引:16
作者
Augusti, Paula Rossini [2 ,3 ]
Ruviaro, Amanda Roggia [1 ]
Quatrin, Andreia [1 ]
Somacal, Sabrina [1 ]
Marafiga Conterato, Greicy Michelle [1 ]
Vicentini, Juliana Tanara [1 ]
Frescura Duarte, Marta Medeiros [4 ]
Emanuelli, Tatiana [1 ]
机构
[1] Univ Fed Santa Maria, Dept Food Technol & Sci, Integrated Ctr Lab Anal Dev NIDAL, Ctr Rural Sci, BR-97119900 Santa Maria, RS, Brazil
[2] Univ Fed Rio Grande do Sul, Dept Biochem, Grad Program Biol Sci Biochem, Inst Hlth Basic Sci, Porto Alegre, RS, Brazil
[3] Fed Univ Pampa, Itaqui, RS, Brazil
[4] Univ Luterana Brasil, Dept Hlth Sci, Santa Maria, RS, Brazil
关键词
Atherogenic index; Hypercholesterolemia; Oxidized low density lipoprotein; Superoxide dismutase; Thioredoxin reductase; CORONARY-ARTERY-DISEASE; THIOREDOXIN REDUCTASE; PARAOXONASE; SERUM; STRESS; CHOLESTEROL; PLASMA; ANION; OXLDL; ASSAY;
D O I
10.1186/1476-511X-11-79
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Background: There is a relationship among hypercholesterolemia, oxidative stress and inflammation in the atherogenesis. Thus, the objective of the present study was to assess paraoxonase (PON1), superoxide dismutase (SOD) and thioredoxin reductase (TrxR-1) activities and their relationship with lipids, oxidative stress and inflammation in subjects with different low density lipoprotein-cholesterol (LDL) levels. Methods: Serum lipids, highly sensitive C-reactive protein (hs-CRP), lipid and protein oxidation, oxidized LDL (LDLox) and LDLox autoantibodies (LDLoxAB) levels and enzymes activities were measured in a total of 116 subjects that were divided into the following groups according to their LDL levels: low-LDL group (LDL < 100 mg/dL, n = 23), intermediate-LDL group (LDL 100-160 mg/dL, n = 50) and high-LDL group (LDL > 160 mg/dL, n = 43). Results: The LDLox and hs-CRP levels increased in the high-LDL group (2.7- and 3.7-fold, respectively), whereas the intermediate and high-LDL groups had higher LDLoxAB (2.2- and 3.1-fold) when compared to low-LDL group (p < 0.05). Similarly, SOD activity, the atherogenic index (AI) and protein oxidation were also higher in the intermediate (1.3-, 1.3- and 1.2-fold) and high-LDL (1.6-, 2.3- and 1.6-fold) groups when compared to the low-LDL group (p < 0.05). Lipid oxidation and SOD/TrxR-1 ratio increased only in the high-LDL group (1.3- and 1.6-fold) when compared to the low-LDL group (p < 0.05). The SOD/TrxR-1 ratio was positively correlated to TBARS (r = 0.23, p < 0.05), LDLox (r = 0.18, p < 0.05), LDLoxAB (r = 0.21, p < 0.05), LDL (r = 0.19, p < 0.05) and AI (r = 0.22, p < 0.05). PON1 and TrxR-1 activities were similar among groups. Conclusions: Some oxidative events initiate when LDL levels are clinically acceptable. Moreover, hypercholesterolemic patients have an imbalance in SOD and TrxR-1 activities that is positively associated to LDL oxidation.
引用
收藏
页数:9
相关论文
共 41 条
[1]   Physiological functions of thioredoxin and thioredoxin reductase [J].
Arnér, ESJ ;
Holmgren, A .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 2000, 267 (20) :6102-6109
[2]   Astaxanthin Reduces Oxidative Stress, but not Aortic Damage in Atherosclerotic Rabbits [J].
Augusti, Paula R. ;
Conterato, Greicy M. M. ;
Somacal, Sabrina ;
Sobieski, Rocheli ;
Quatrin, Andreia ;
Maurer, Luana ;
Rocha, Marta P. ;
Denardin, Ione T. ;
Emanuelli, Tatiana .
JOURNAL OF CARDIOVASCULAR PHARMACOLOGY AND THERAPEUTICS, 2009, 14 (04) :314-322
[3]  
Augusti PR, J CLIN BIOC IN PRESS, DOI [10.3164/jcbn.11-74., DOI 10.3164/JCBN]
[4]   Does paraoxonase play a role in susceptibility to cardiovascular disease? [J].
Aviram, M .
MOLECULAR MEDICINE TODAY, 1999, 5 (09) :381-386
[5]   Human serum paraoxonase (PON 1) is inactivated by oxidized low density lipoprotein and preserved by antioxidants [J].
Aviram, M ;
Rosenblat, M ;
Billecke, S ;
Erogul, J ;
Sorenson, R ;
Bisgaier, CL ;
Newton, RS ;
La Du, B .
FREE RADICAL BIOLOGY AND MEDICINE, 1999, 26 (7-8) :892-904
[6]   Paraoxonase inhibits high-density lipoprotein oxidation and preserves its functions - A possible peroxidative role for paraoxonase [J].
Aviram, M ;
Rosenblat, M ;
Bisgaier, CL ;
Newton, RS ;
Primo-Parmo, SL ;
La Du, BN .
JOURNAL OF CLINICAL INVESTIGATION, 1998, 101 (08) :1581-1590
[7]  
Bachorik PS, 1996, METHOD ENZYMOL, V129, P78
[8]   Animal model of autism induced by prenatal exposure to valproate: Behavioral changes and liver parameters [J].
Bambini-Junior, Victorio ;
Rodrigues, Leticia ;
Behr, Guilherme Antonio ;
Fonseca Moreira, Jose Claudio ;
Riesgo, Rudimar ;
Gottfried, Carmem .
BRAIN RESEARCH, 2011, 1408 :8-16
[9]  
Batlouni M, 1997, Arq Bras Cardiol, V68, P55
[10]   Effects of atorvastatin therapy on hypercholesterolernic rabbits with respect to oxidative stress, nitric oxide pathway and homocysteine [J].
Bolayirli, Ibrahim Murat ;
Aslan, Mahmure ;
Balci, Huriye ;
Altug, Tuncay ;
Hacibekiroglu, Munire ;
Seven, Arzu .
LIFE SCIENCES, 2007, 81 (02) :121-127