Integrative mRNA-miRNA interaction analysis associate with immune response of sea cucumber Apostichopus japonicus based on transcriptome database

被引：22
作者：
Zhou, Xiaoxu [1 ]
Chang, Yaqing [1 ]
Zhan, Yaoyao [1 ]
Wang, Xiuli [1 ]
Lin, Kai [1 ]
机构：
[1] Dalian Ocean Univ, Minist Agr, Key Lab Mariculture & Stock Enhancement North Chi, Dalian 116023, Peoples R China
来源：
FISH & SHELLFISH IMMUNOLOGY | 2018年 / 72卷
基金：
中国国家自然科学基金;
关键词：
Sea cucumber (Apostichopus japonicus); Vthrio splendidus; rnRNA-miRNA interaction; GO analysis; Temporal expression; C-TYPE LECTIN; EXPRESSION ANALYSIS; MOLECULAR-CLONING; GENE; IDENTIFICATION; MICRORNAS; ALPHA-2-MACROGLOBULIN; DISCOVERY; DISEASE; TARGETS;
D O I：
10.1016/j.fsi.2017.10.031
中图分类号：
S9 [水产、渔业];
学科分类号：
0908 ;
摘要：
MicroRNAs (miRNAs) constitute a family of endogenous non-coding small RNAs that have been demonstrated to be the key effectors in mediating host-pathogen interactions. Additionally, high-throughput sequencing provides unexampled opportunities to identify the pathogenic mechanism underlying miRNAs. In the present study, the target genes of immune-related miRNAs (miR-31, miR-2008, miR-92a, miR-210 and miR-7) and specific miRNAs (miR-2004) in Echinodermata were predicted in silico and validated. Gene ontology (GO) analysis of the target genes of these six miRNAs were conducted to further understand the regulatory function in the host immunity of Apostichopus japonicus (A. japonicus). Among the putative target genes of the six miRNAs, various immune related targets were annotated, such as Nephl, SECI4L1, p105, GL2, LYS, FNL4L, mTOR, LITAF, SLC44, TLR3, Apaf-1, and CNTN4. This work will provide valuable genetic resources to understand the interaction of multiple mRNA-miRNAs and the regulation mechanism in the anti-bacterial process in the sea cucumber.
引用
收藏
页码：69 / 76
页数：8
相关论文
共 52 条
[1]   Shrimp Alpha-2-Macroglobulin Prevents the Bacterial Escape by Inhibiting Fibrinolysis of Blood Clots [J].
Chaikeeratisak, Vorrapon ;
Somboonwiwat, Kunlaya ;
Tassanakajon, Anchalee .
PLOS ONE, 2012, 7 (10)
[2]   Genetic variability analysis in five populations of the sea cucumber Stichopus (Apostichopus) japonicus from China, Russia, South Korea and Japan as revealed by microsatellite markers [J].
Chang, Yaqing ;
Feng, Zhigang ;
Yu, Jiaping ;
Ding, Jun .
MARINE ECOLOGY-AN EVOLUTIONARY PERSPECTIVE, 2009, 30 (04) :455-461
[3]  
Chen J., 2004, ADV SEA CUCUMBER AQU, P25
[4]   Differential toll-like receptor 3 (TLR3) expression and apoptotic response to TLR3 agonist in human neuroblastoma cells [J].
Chuang, Jiin-Haur ;
Chuang, Hui-Ching ;
Huang, Chao-Cheng ;
Wu, Chia-Ling ;
Du, Yung-Ying ;
Kung, Mei-Lang ;
Chen, Chih-Hao ;
Chen, San-Cher ;
Tai, Ming-Hong .
JOURNAL OF BIOMEDICAL SCIENCE, 2011, 18
[5]   Purification, characterization and molecular cloning of alpha-2-macroglobulin in cobia, Rachycentron canadum [J].
Chuang, Wen-Hsiao ;
Liu, Ping-Chung ;
Hung, Chia-Yu ;
Lee, Kuo-Kau .
FISH & SHELLFISH IMMUNOLOGY, 2014, 41 (02) :346-355
[6]   Isolation and pathogenicity of pathogens from skin ulceration disease and viscera ejection syndrome of the sea cucumber Apostichopus japonicuss [J].
Deng, Huan ;
He, Chongbo ;
Zhou, Zunchun ;
Liu, Chang ;
Tan, Kefei ;
Wang, Nianbin ;
Jiang, Bei ;
Gao, Xianggang ;
Liu, Weidong .
AQUACULTURE, 2009, 287 (1-2) :18-27
[7]  
Dong Y., 2005, Fish. Sci., V24, P4
[8]  
Egoiov A. V., 2003, PRIKL BIOKHIMIIA MIK, V39, P452
[9]   Global miRNA expression analysis of serous and clear cell ovarian carcinomas identifies differentially expressed miRNAs including miR-200c-3p as a prognostic marker [J].
Elgaaen, Bente Vilming ;
Olstad, Ole Kristoffer ;
Haug, Kari Bente Foss ;
Brusletto, Berit ;
Sandvik, Leiv ;
Staff, Anne Cathrine ;
Gautvik, Kaare M. ;
Davidson, Ben .
BMC CANCER, 2014, 14
[10]   Molecular cloning, characterization and expression analysis of a C-type lectin (AJCTL) from the sea cucumber Apostichopus japonicus [J].
Han, Lu-Lu ;
Yuan, Zhe ;
Dahms, Hans-Uwe ;
Li, Qiu-Ying ;
Zhang, Qiao-Zhi ;
Wu, Rong-Jie ;
Tan, Jian ;
Zou, Xiang-Yang ;
Hou, Lin .
IMMUNOLOGY LETTERS, 2012, 143 (02) :137-145
123456