Static Magnetic Stimulation Induces Cell-type Specific Alterations in the Viability of SH-SY5Y Neuroblastoma Cell Line

被引：5
作者：
Medeiros, Helouise R. [1 ,2 ,3 ]
Assumpcao, Jose A. F. [1 ,2 ]
Medeiros, Liciane F. [2 ]
Stapenhorst, Martina [3 ]
Nunes, Lara [3 ]
Henckes, Nicole A. C. [3 ]
Cruz, Carolina Uribe [4 ]
Fregni, Felipe [5 ,6 ,7 ]
Sanches, Paulo R. S. [8 ]
Oliveira, Fernanda S. O. [3 ]
Caumo, Wolnei [1 ,2 ]
Cirne-Lima, Elizabeth O. [3 ]
Torres, Iraci L. S. [1 ,2 ]
机构：
[1] Univ Rio Grande Sul UFRGS, Fac Med, PostGrad Program Med Med Sci, Porto Alegre, RS, Brazil
[2] Hosp Clin Porto Alegre, Expt Res Ctr, Translat Nucleus Pain Pharmacol & Neuromodulat, Porto Alegre, RS, Brazil
[3] Hosp Clin Porto Alegre, Expt Res Ctr, Embryol & Cell Differentiat Lab, Porto Alegre, RS, Brazil
[4] Hosp Clin Porto Alegre, Expt Res Ctr, Expt Lab Hepatol & Gastroenterol, Porto Alegre, RS, Brazil
[5] Spaulding Rehabil Hosp, Dept Phys Med & Rehabil, Lab Neuromodulat, Boston, MA USA
[6] Harvard Med Sch, Massachusetts Gen Hosp, Boston, MA 02115 USA
[7] Harvard Med Sch, Ctr Noninvas Brain Stimulat, Beth Israel Deaconess Med Ctr, Boston, MA 02115 USA
[8] Hosp Clin Porto Alegre, Biomed Engn Lab, Res & Post Grad Grp, Porto Alegre, RS, Brazil
来源：
ANTICANCER RESEARCH | 2020年 / 40卷 / 09期
关键词：
Static magnetic stimulation; neuroblastoma; cell viability; cell death; cell cycle; SOMATOSENSORY-EVOKED POTENTIALS; FIELD STIMULATION; ALZHEIMERS-DISEASE; CORTEX; DIFFERENTIATION; DEPRESSION; INCREASES; MECHANISM; CISPLATIN; BDNF;
D O I：
10.21873/anticanres.14518
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Background/Aim: Magnetic stimulation is used in the treatment of a diversity of diseases, but a complete understanding of the underlying mechanisms of action requires further investigation. We examined the effect of static magnetic stimulation (SMS) in different cell lines. Materials and Methods: A culture plate holder with attached NeFeB magnets was developed. Different magnetic field intensities and periods were tested in tumoral and non-tumoral cell lines. To verify the cellular responses to SMS, cell viability, cell death, cell cycle and BDNF expression were evaluated. Results: Exposure of SH-SY5Y cells to SMS for 24 hours led to a decrease in cell viability. Analysis 24 h after stimulation revealed a decrease in apoptotic and double-positive cells, associated with an increase in the number of necrotic cells. Conclusion: The effects of SMS on cell viability are cell type-specific, inducing a decrease in cell viability in SH-SY5Y cells. This suggests that SMS may be a potential tool in the treatment of neuronal tumors.
引用
收藏
页码：5151 / 5158
页数：8
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