Characteristics of protein partitioning in an aqueous micellar-gel system

被引:2
作者
van den Broeke, LJP
van Roosmalen, D
Dohmen-Speelmans, MPJ
Dietz, CHJT
van der Wielen, LAM
Keurentjes, JTF
机构
[1] Eindhoven Univ Technol, Proc Dev Grp, Dept Chem Engn & Chem, NL-5600 MB Eindhoven, Netherlands
[2] Delft Univ Technol, Dept Biotechnol, NL-2628 BC Delft, Netherlands
关键词
nonionic surfactant; aqueous micellar-gel systems; size exclusion; excluded-volume interactions; protein purification; sephacryl large pore gel;
D O I
10.1002/bit.20710
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Partitioning of proteins has been studied experimentally in a system combining a gel-bead phase and a nonionic micellar phase. The micellar phase consists of cylindrically shaped micelles, which are completely excluded from the gel-bead phase. Partitioning of single-component protein solutions (myoglobin, ovalbumin, and BSA) is determined by excluded-volume interactions in the micellar phase, and as a result the proteins prefer the gel-bead phase to the micellar phase. The protein concentration inside the gel beads increases with an increase in volume fraction of the micelles and increases with an increase in the size of the proteins. The protein partition coefficients obtained for a binary mixture of myoglobin and bovine serum albumin (BSA) show the same protein concentration dependence as the single component protein partition coefficients. (c) 2005 Wiley Periodicals, Inc.
引用
收藏
页码:355 / 360
页数:6
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