Fast microscopical dissection of action scenes played by Escherichia coli RNA polymerase

被引：16

作者：

Suzuki, Yuki ^{[1
]}

Shin, Minsang ^{[1
]}

Yoshida, Aiko ^{[1
]}

Yoshimura, Shige H. ^{[1
]}

Takeyasu, Kunio ^{[1
]}

机构：

[1] Kyoto Univ, Grad Sch Biostudies, Lab Plasma Membrane & Nucl Signaling, Sakyo Ku, Kyoto 6068501, Japan

来源：

FEBS LETTERS | 2012年 / 586卷 / 19期

基金：

日本学术振兴会;

关键词：

Atomic force microscopy; Fast-scanning atomic force microscopy; RNA polymerase; Protein-DNA interaction; ATOMIC-FORCE MICROSCOPY; CONFORMATIONAL-CHANGES; DNA INTERACTIONS; START SITE; REAL-TIME; TRANSCRIPTION; COMPLEXES; VISUALIZATION; DIFFUSION; REVEALS;

D O I：

10.1016/j.febslet.2012.06.033

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Using fast-scanning atomic force microscopy, we directly visualized the interaction of Escherichia coli RNA polymerase (RNAP) with DNA at the scan rate of 1-2 frames per second. The analyses showed that the RNAP can locate the promoter region not only by sliding but also by hopping and/or segmental transfer. Upon the addition of 0.05 mM NTPs to the stalled complex, the RNAP molecule pulled the template DNA uni-directionally at the rates of 15 nucleotides/s on average. The present method is potentially applicable to examine a variety of protein-nucleic acid interactions, especially those involved in the process of gene regulation. (C) 2012 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：3187 / 3192

页数：6

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