Ovarian contrast-enhanced ultrasonography and Doppler fluxometry in bitches during the postovulatory estrus and corpora lutea formation

被引:5
作者
Aires, Luiz Paulo Nogueira [1 ]
Gasser, Beatriz [2 ]
Silva, Priscila [2 ]
Del'Aguila-Silva, Priscila [1 ]
Yamada, Diego Iwao [3 ]
Carneiro, Rafael Kretzer [2 ]
Lima, Bruna Bruna Bressianini [2 ]
Padilha-Nakaghi, Luciana Cristina [1 ]
Uscategui, Ricardo Andres Ramirez [4 ]
Spada, Stefano [5 ]
Russo, Marco [5 ]
Feliciano, Marcus Antonio Rossi [1 ,2 ,6 ]
机构
[1] Sao Paulo State Univ Julio Mesquita Filho FCAV UN, Sch Agr & Vet Sci, Dept Pathol Reprod & One Hlth, Sao Paulo, Brazil
[2] Sao Paulo State Univ Julio Mesquita Filho FCAV UN, Sch Agr & Vet Sci, Dept Vet Clin & Surg, Jaboticabal, SP, Brazil
[3] Marilia Univ UNIMAR, Sao Paulo, Brazil
[4] Univ CES, Fac Vet Med & Anim Sci, Res Grp INCA CES, Medellin, Colombia
[5] Univ Naples Federico II, Dept Vet Med & Anim Prod, Naples, Italy
[6] Sao Paulo Univ USP, Fac Anim Sci & Food Engn FZEA, Lab Vet Imaginol, Pirassununga, SP, Brazil
基金
巴西圣保罗研究基金会;
关键词
Canine; ovarian perfusion; ovarian follicle; corpus luteum; angiogenesis; CYCLIC CHANGES; BLOOD-FLOW; ULTRASOUND; ANGIOGENESIS; PERFUSION; US;
D O I
10.1016/j.theriogenology.2022.10.009
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
The aim of the present study was to evaluate the vascularization features of canine ovaries during the follicular phase and the formation of the corpora lutea by using Doppler ultrasonography and Contrast -Enhanced Ultrasound (CEUS). Eight healthy bitches were enrolled in the study and were evaluated at five different timepoints (T1 -T5) of the estrous cycle established by vaginal cytology and serum proges-terone concentration. Ultrasonographic examinations were performed by a single operator using the ACUSON S2000/SIEMENS machine equipped with a linear multifrequency transducer (9.0 MHz). Color -coded Doppler evaluation of the ovarian parenchyma was performed to investigate the aspects of the signal detection throughout the different timepoints. Pulsed-wave Doppler of the intraovarian arteries was performed to evaluate spectral waveform and doppler velocimetric parameters of Systolic Peak Velocity (SPV cm/s), End Diastolic Velocity (EDV cm/s), Resistivity Index (RI) and Pulsatility Index (PI). CEUS evaluation of the ovaries was performed using a vascular contrast agent (SonoVue (R), Bracco, Sao Paulo, Brazil) and the CADENCETM Contrast Pulse Sequencing (CPS, Siemens) software, in order to perform both qualitative and quantitative analysis. Perfusion parameters of pixel number, peak intensity (PPI in %), time to peak intensity (TTP in s), mean transit time (MTT in s) and area under the curve (AUC in %). Colour-coded Doppler evaluation demonstrated an increase in signal detection as cycle progressed, with blood flow initially detected with few coloured pixels and mainly at the ventral surface of the ovaries. Further on, the number of coloured pixels increased and spreading to the central region, resulting in a circular-like pattern around the corpora hemorrhagica. The spectral waveform was consistent at all timepoints. SPV (cm/s) and EDV (cm/s) presented a numeric trend and a slight statistical difference at all timepoints, whereas no difference was found for RI and PI. CEUS evaluation demon-strated an increase in pixel intensity across all the timepoints. Quantitative CEUS analysis revealed a statistical difference in PPI (%), MTT (s) and AUC (%) at T5. CEUS evaluation of the ovaries was feasible and demonstrated a marked increase in perfusion parameters in the late postovulatory period, demonstrating its applicability in the assessment of canine corpora lutea development. (c) 2022 Elsevier Inc. All rights reserved.
引用
收藏
页码:162 / 170
页数:9
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