Small-conductance calcium-activated potassium current modulates the ventricular escape rhythm in normal rabbit hearts

BACKGROUND The apamin- sensitive small- conductance calciumactivated K (SK) current IKAS modulates automaticity of the sinus node. IKAS blockade by apamin causes sinus bradycardia. OBJECTIVE The purpose of this study was to test the hypothesis that IKAS modulates ventricular automaticity. METHODS We tested the effects of apamin (100 nM) on ventricular escape rhythms in Langendorff- perfused rabbit ventricles with atrioventricular block (protocol 1) and on recorded transmembrane action potential of pseudotendons of superfused right ventricular endocardial preparations (protocol 2). RESULTS All preparations exhibited spontaneous ventricular escape rhythms. In protocol 1, apamin decreased the atrial rate from 186.2 +/- 18.0 bpm to 163.8 +/- 18.7 bpm (N = 6; P = .006) but accelerated the ventricular escape rate from 51.5 +/- 10.7 bpm to 98.2 +/- 25.4 bpm (P= .031). Three preparations exhibited bursts of nonsustained ventricular tachycardia and pauses, resulting in repeated burst termination pattern. In protocol 2, apamin increased the ventricular escape rate from 70.2 +/- 13.1 bpm to 110.1 +/- 2.2 bpm (P = .035). Spontaneous phase 4 depolarization was recorded from the pseudotendons in 6 of 10 preparations at baseline and in 3 in the presence of apamin. There were no changes of phase 4 slope (18.37 +/- 3.55 mV/s vs 18.93 +/- 3.26 mV/s, N = 3; P = .231,), but the threshold of phase 0 activation (mV) reduced from - 67.97 +/- 1.53 to - 75.26 +/- 0.28 (P = .034). Addition of JTV- 519, a ryanodine receptor 2 stabilizer, in 5 preparations reduced escape rate back to baseline. CONCLUSION Contrary to its bradycardic effect in the sinus node, I-KAS blockade by apamin accelerates ventricular automaticity and causes repeated nonsustained ventricular tachycardia in normal ventricles. ryanodine receptor 2 blockade reversed the apamin effects on ventricular automaticity.