The effect of feeding maize silage 1 h or 9 h before the herbage meal on dry matter intake, milk production, nitrogen partitioning and rumen function of lactating dairy cows

The study was conducted to evaluate the effect of the timing of feeding maize silage before a short herbage meal on dry matter (DM) intake, milk production, nitrogen (N) partitioning and rumen function of lactating dairy cows. Ten lactating, pregnant, Friesian x Jersey rumen-fistulated dairy cows aged 4-5 years were blocked in pairs by milksolids (MS; sum of protein and fat) production (1.73 +/- 0.097 kg MS/cow. day; mean +/- s.d.), bodyweight (519 +/- 24.7 kg), days in milk (163 +/- 12.5), and body condition score (3.9 +/- 0.21). Cows within pairs were randomly allocated to one of two treatments: (1) five cows were offered one-third of their estimated total DM intake (5 kg/cow. day) as maize silage after the afternoon milking at 1500 hours, similar to 1 h before herbage was provided (1BH); (2) five cows were offered one-third of their estimated total DM intake as maize silage after morning milking at 0700 hours, similar to 9 h before herbage was provided (9BH). All cows were offered two-thirds of their total estimatedDMintake (11 kg/cow. day) over a period of 4 h from 1600 hours to 2000 hours as freshly cut perennial ryegrass (Lolium perenne L.) and white clover (Trifolium repens) in an indoor system. Cows were adapted to diets for 9 days (Days 1-9), followed by 5 days of measurement (Days 10-14). Maize silage DM intake did not differ between treatments averaging 5.1 kg DM/cow.day. Herbage DM intake was greater (P = 0.02) for 9BH (11.2 kg DM/cow.day) than 1BH (10.4 kg DM/cow.day). Milk yield (P = 0.001) and MS production (P = 0.08) were greater for 9BH than 1BH (18.8 vs 15.4 kg/cow. day and 1.63 vs 1.48 kg MS/cow. day, respectively). There was no effect of time of supplementation on N partitioned to milk, faeces, or urine. The purine derivatives to creatinine ratio, as an indication of microbial protein production, was greater (P = 0.04) for 9BH than 1BH. Daily mean rumen pH and the concentration of total rumen volatile fatty acids, acetate, butyrate, and ammonia N were not affected by treatment. However, the peak concentration of ruminal ammonia N was 27% lower (P < 0.05) for 9BH than 1BH. The concentration of non-esterified fatty acid (P = 0.03) and beta-hydroxy butyric acid (P = 0.01) were greater for 1BH than 9BH (0.14 vs 0.09 and 0.81 vs 0.71 mmol/L, respectively). These results indicate that under herbage feed restriction, altering the time of supplementation relative to the herbage meal can increase herbage DM intake and subsequent milk production.