In vitro regeneration of plantlets from seedlings explants of guava (Psidium guajava']java L.) cv. Safeda

Regeneration of plantlets from In vitro germinated seedling explants was carried out at NIFA, Peshawar. Multiple shoots were generated from hi vitro germinated (50 days-old) seedlings of Psidium guajava L. Cv. Safeda. Modified Murashige & Skoog (MMS) medium Supplemented with 6-benzylaminopurine (BAP), Zeatin and Gibberelic acid (GA(3)), Zeatin 1.0 mg/L combined with GA(3) 0.5 mg/L gave the highest response (47.6%) with the regeneration of (3.2) shoots per original explants. Each shoot gave rise to about 4.4 shoots per explant upon sub culture on MMS supplemented with BAP 1.0 mg/L combined with Kinetin 0.5 mg/L. The micro shoots were successfully rooted oil 1/2 strength MMS medium supplemented with IBA 1.5 mg/L and NAA 0.5 mg/L. where 85% rooted plantlets were obtained. The In vitro plantlets after rooting were acclimatized in Jiffy pots and established in the soil.