Evaluation of Bcl-2, Bcl-x and Cleaved Caspase-3 in Malignant Peripheral Nerve Sheath Tumors and Neurofibromas

被引：8
作者：
Cunha, Karin S. [1 ]
Caruso, Anabela C. [2 ]
Faria, Paulo A. [3 ]
Da Silva, Licinio E. [4 ]
Da Fonseca, Eliene C. [1 ]
Geller, Mauro [5 ]
De Moura-Neto, Rodrigo S. [6 ]
Lopes, Vania S. [1 ]
机构：
[1] Univ Fed Fluminense, Hosp Univ Antonio Pedro, Programa Posgrad Patol, BR-24033090 Niteroi, RJ, Brazil
[2] Inst Nacl Traumatol & Ortopedia, Serv Anat Patol, BR-20940070 Rio De Janeiro, RJ, Brazil
[3] Inst Nacl Canc, Div Pathol, BR-20220400 Rio De Janeiro, RJ, Brazil
[4] Univ Fed Fluminense, Dept Estat, Inst Matemat & Estat, BR-24020140 Niteroi, RI, Brazil
[5] Ctr Univ Serra Orgaos UNIFESO, Dept Microbiol & Immunol, BR-25964004 Teresopolis, RJ, Brazil
[6] Univ Fed Rio de Janeiro, Inst Biol, BR-21941902 Rio De Janeiro, Brazil
来源：
ANAIS DA ACADEMIA BRASILEIRA DE CIENCIAS | 2013年 / 85卷 / 04期
关键词：
apoptosis regulatory proteins; malignant peripheral nervous sheath tumors; neurofibroma; Neurofibromatosis; 1; GROWTH-HORMONE RECEPTOR; SOFT-TISSUE SARCOMAS; CELL LUNG-CANCER; IMMUNOHISTOCHEMICAL ANALYSIS; BREAST-CANCER; PROGNOSTIC VALUE; PROTEIN EXPRESSION; SCHWANN-CELLS; P53; PROTEIN; IN-VIVO;
D O I：
10.1590/0001-3765201320120026
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
AIMS: To study the expression of Bcl-2, Bcl-x, as well the presence of cleaved caspase-3 in neurofibromas and malignant peripheral nerve sheath tumors. The expression of Bcl-2 and Bcl-x and the presence of cleaved caspase 3 were compared to clinicopathological features of malignant peripheral nerve sheath tumors and their impact on survival rates were also investigated. MATERIALS AND METHODS: The evaluation of Bcl-2, Bcl-x and cleaved caspase-3 was performed by immunohistochemistry using tissue microarrays in 28 malignant peripheral nerve sheath tumors and 38 neurofibromas. Immunoquantification was performed by computerized digital image analysis. CONCLUSIONS: Apoptosis is altered in neurofibromas and mainly in malignant peripheral nerve sheath tumors. High levels of cleaved caspase-3 are more common in tumors with more aggressive histological features and it is associated with lower disease free survival of patients with malignant peripheral nerve sheath tumors.
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收藏
页码：1497 / 1511
页数：15
相关论文
共 49 条
[1]   Immunohistochemical analysis of cleaved caspase-3 detects high level of apoptosis frequently in diffuse large B-cell lymphomas of the central nervous system [J].
Arai, M ;
Sasaki, A ;
Saito, N ;
Nakazato, Y .
PATHOLOGY INTERNATIONAL, 2005, 55 (03) :122-129
[2]  
Asselin E, 2001, CANCER RES, V61, P1862
[3]   c-Myb and Bcl-x overexpression predicts poor prognosis in colorectal cancer -: Clinical and experimental findings [J].
Biroccio, A ;
Benassi, B ;
D'Agnano, I ;
D'Angelo, C ;
Buglioni, S ;
Mottolese, M ;
Ricciotti, A ;
Citro, G ;
Cosimelli, M ;
Ramsay, RG ;
Calabretta, B ;
Zupi, G .
AMERICAN JOURNAL OF PATHOLOGY, 2001, 158 (04) :1289-1299
[4]   Bcl-2 Modulation to Activate Apoptosis in Prostate Cancer [J].
Bray, Kevin ;
Chen, Hsin-Yi ;
Karp, Cristina M. ;
May, Michael ;
Ganesan, Shridar ;
Karantza-Wadsworth, Vassiliki ;
DiPaola, Robert S. ;
White, Eileen .
MOLECULAR CANCER RESEARCH, 2009, 7 (09) :1487-1496
[5]   Malignant peripheral nerve sheath tumors: clinicopathological aspects, expression of p53 and survival [J].
Cunha, Karin S. G. ;
Caruso, Anabela C. ;
de Faria, Paulo A. S. ;
da Silva, Licinio E. ;
Pires, Andrea R. C. ;
Geller, Mauro ;
Lopes, Vania S. ;
de Moura-Neto, Rodrigo S. .
CLINICS, 2012, 67 (08) :963-968
[6]   Identification of growth hormone receptor in localised neurofibromas of patients with neurofibromatosis type 1 [J].
Cunha, KSG ;
Barboza, EP ;
da Fonseca, EC .
JOURNAL OF CLINICAL PATHOLOGY, 2003, 56 (10) :758-763
[7]   Apoptosis and expression of its regulatory proteins in soft tissue sarcomas [J].
Dan'ura, T ;
Kawai, A ;
Morimoto, Y ;
Naito, N ;
Yoshida, A ;
Inoue, H .
CANCER LETTERS, 2002, 178 (02) :167-174
[8]   Neurofibromin regulates neural stem cell proliferation, survival, and astroglial differentiation in vitro and in vivo [J].
Dasgupta, B ;
Gutmann, DH .
JOURNAL OF NEUROSCIENCE, 2005, 25 (23) :5584-5594
[9]  
DUCATMAN BS, 1986, CANCER-AM CANCER SOC, V57, P2006, DOI 10.1002/1097-0142(19860515)57:10<2006::AID-CNCR2820571022>3.0.CO
[10]  
2-6
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