Oligonucleotide conjugates of Nile Red

被引：6
|
作者：
Prokhorenko, IA [1 ]
Dioubankova, NN [1 ]
Korshun, VA [1 ]
机构：
[1] Russian Acad Sci, Shemyakin Ovchinnikov Inst Bioorgan Chem, Moscow 117997, Russia
来源：
NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS | 2004年 / 23卷 / 1-2期
基金：
俄罗斯基础研究基金会;
关键词：
modified oligonucleotides; Nile Red; interaction with ds DNA;
D O I：
10.1081/NCN-120028344
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Oligonucleotides containing long chain amine on uridine-2'-carbamate have been covalently modified with Nile Red, a hydrophobic long wavelength fluorescent benzophenoxazine dye. The fluorescence of the dye is quenched in oligonucleotide conjugates. Thermal denaturation studies show considerable interactions of Nile Red with DNA-DNA duplexes.
引用
收藏
页码：509 / 520
页数：12
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