Rapid Analysis of Components in Coptis chinensis Franch by Ultra-Performance Liquid Chromatography with Quadrupole Time-of-Flight Mass Spectrometry

被引：42

作者：

Tian, Peng-peng ^{[1
,2
]}

Zhang, Xiao-xu ^{[1
,2
]}

Wang, Hong-ping ^{[1
]}

Li, Pu-ling ^{[1
,3
]}

Liu, Yu-xin ^{[2
,4
]}

Li, Shao-jing ^{[1
]}

机构：

[1] China Acad Chinese Med Sci, Inst Chinese Mat Med, Beijing, Peoples R China

[2] Hebei Univ, Coll Pharmaceut Sci, Lab Cell Pharmacol, Baoding, Peoples R China

[3] Henan Univ Tradit Chinese Med, Zhengzhou, Peoples R China

[4] Hebei Univ, Drug Qual Control Key Lab Hebei Prov, Baoding, Peoples R China

来源：

PHARMACOGNOSY MAGAZINE | 2017年 / 13卷 / 49期

基金：

中国国家自然科学基金;

关键词：

Alkaloids; Coptis chinensis French; non-alkaloids; Q-TOF; UPLC; ALKALOIDS;

D O I：

10.4103/0973-1296.197635

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

Background: Coptis chinensis Franch is a traditional Chinese medical herb. Objective: In this article, ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry was used to rapidly, qualitatively, and comprehensively identify the components in Coptis chinensis Franch. Materials and Methods: Chromatographic separation was achieved on an Agilent Zorbax RRHD Eclipse Plus C-18 column. The mobile phase consisted of 0.1% formic acid water (A) and 0.1% formic acid acetonitrile (B) with a gradient program. Qualitative analysis was performed on an Agilent 6540 quadrupole time-of-flight mass spectrometer, which was equipped with a Dual AJS ESI source operating in negative mode. Results: A total of 30 alkaloid and non-alkaloid components of Coptis chinensis Franch were identified in only 14 min. Conclusion: This study helped to provide a basis for the quality control of Coptis chinensis Franch.

引用

页码：175 / 179

页数：5

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