Isolation and characterisation of CD9-positive pituitary adult stem/progenitor cells in rats

被引：25
作者：
Horiguchi, Kotaro [1 ,2 ]
Fujiwara, Ken [3 ]
Yoshida, Saishu [2 ]
Nakakura, Takashi [4 ]
Arae, Ken [5 ]
Tsukada, Takehiro [6 ]
Hasegawa, Rumi [1 ]
Takigami, Shu [1 ]
Ohsako, Shunji [1 ]
Yashiro, Takashi [3 ]
Kato, Takako [2 ]
Kato, Yukio [2 ,7 ]
机构：
[1] Kyorin Univ, Dept Hlth Sci, Lab Anat & Cell Biol, 5-4-1 Shimorenjaku, Mitaka, Tokyo 1818612, Japan
[2] Meiji Univ, Inst Reprod & Endocrinol, Tama Ku, 1-1-1 Higashimita, Kawasaki, Kanagawa 2148571, Japan
[3] Jichi Med Univ, Sch Med, Dept Anat, Div Histol & Cell Biol, 3311-1 Yakushiji, Shimotsuke, Tochigi 3290498, Japan
[4] Teikyo Univ, Grad Sch Med, Dept Anat, Itabashi Ku, 2-11-1 Kaga, Tokyo 1738605, Japan
[5] Kyorin Univ, Dept Hlth Sci, Lab Immunol, 5-4-1 Shimorenjaku, Mitaka, Tokyo 1818612, Japan
[6] Toho Univ, Fac Sci, Dept Biomol Sci, 2-2-1 Miyama, Funabashi, Chiba 2748510, Japan
[7] Meiji Univ, Sch Agr, Dept Life Sci, Tama Ku, 1-1-1 Higashi Mita, Kawasaki, Kanagawa 2148571, Japan
来源：
SCIENTIFIC REPORTS | 2018年 / 8卷
关键词：
GREEN FLUORESCENT PROTEIN; FOLLICULO-STELLATE CELLS; ANTERIOR-PITUITARY; STEM-CELLS; S100-BETA-POSITIVE CELLS; TRANSGENIC RAT; IDENTIFICATION; PROLIFERATION; HOMEOSTASIS; PARENCHYMA;
D O I：
10.1038/s41598-018-23923-0
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
S100 beta protein and SOX2-double positive (S100 beta/SOX2-positive) cells have been suggested to be adult pituitary stem/progenitor cells exhibiting plasticity and multipotency. The aim of the present study was to isolate S100 beta/SOX2-positive cells from the adult anterior lobes of rats using a specific antibody against a novel membrane marker and to study their characteristics in vitro. We found that cluster of differentiation (CD) 9 is expressed in the majority of adult rat S100 beta/SOX2-positive cells, and we succeeded in isolating CD9-positive cells using an anti-CD9 antibody with a pluriBead-cascade cell isolation system. Cultivation of these cells showed their capacity to differentiate into endothelial cells via bone morphogenetic protein signalling. By using the anterior lobes of prolactinoma model rats, the localisation of CD9-positive cells was confirmed in the tumour-induced neovascularisation region. Thus, the present study provides novel insights into adult pituitary stem/progenitor cells involved in the vascularisation of the anterior lobe.
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页数：15
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