Porous hyaluronic acid/sodium alginate composite scaffolds for human adipose-derived stem cells delivery

被引：18
作者：
Son, Yun-Jeong [1 ,2 ]
Yoon, In-Soo [3 ,4 ]
Sung, Jong-Hyuk [5 ]
Cho, Hyun-Jong [6 ]
Chung, Suk-Jae [1 ,2 ]
Shim, Chang-Koo [1 ,2 ]
Kim, Dae-Duk [1 ,2 ]
机构：
[1] Seoul Natl Univ, Coll Pharm, Seoul 151742, South Korea
[2] Seoul Natl Univ, Pharmaceut Sci Res Inst, Seoul 151742, South Korea
[3] Mokpo Natl Univ, Coll Pharm, Jeonnam 534729, South Korea
[4] Mokpo Natl Univ, Nat Med Res Inst, Jeonnam 534729, South Korea
[5] CHA Univ, Dept Appl Biosci, Seoul 135081, South Korea
[6] Kangwon Natl Univ, Coll Pharm, Chunchon 200701, South Korea
来源：
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2013年 / 61卷
基金：
新加坡国家研究基金会;
关键词：
Chondrogenic differentiation; Human adipose-derived stem cells; Hyaluronic acid; Interpenetrating polymeric network; Sodium alginate; INTERPENETRATING POLYMER NETWORKS; ARTICULAR-CARTILAGE REPAIR; CHONDROGENIC DIFFERENTIATION; ACID SCAFFOLD; BONE-MARROW; TISSUE; CHONDROCYTES; BEADS; TRANSPLANTATION; PHENOTYPE;
D O I：
10.1016/j.ijbiomac.2013.06.023
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The aim of this study is to evaluate the feasibility of hyaluronic acid/sodium alginate (HA/SA) scaffold-based interpenetrating polymeric network (IPN) for the proliferation and chondrogenic differentiation of the human adipose-derived stem cells (hADSCs). The hADSCs cultured in HA/SA IPN scaffold exhibited enhanced cell adhesion and proliferation compared to the HA scaffold. Superior chondrogenic differentiation of hADSCs in HA/SA IPN scaffold, compared to HA-based scaffold, was confirmed by measuring expression levels of chondrogenic markers. These results suggested that HA/SA IPN scaffold could provide a desirable environment for the cell adhesion, proliferation and chondrogenic differentiation of hADSCs. (C) 2013 Elsevier B.V. All rights reserved.
引用
收藏
页码：175 / 181
页数：7
相关论文
共 53 条
  • [1] Allemann M, 2001, J BIOMED MATER RES, V55, P13
  • [2] Isolation of human mesenchymal stem cells from bone and adipose tissue
    Bernacki, Susan H.
    Wall, Michelle E.
    Loboa, Elizabeth G.
    [J]. STEM CELL CULTURE, 2008, 86 : 257 - 278
  • [3] REEXPRESSION OF CARTILAGE-SPECIFIC GENES BY DEDIFFERENTIATED HUMAN ARTICULAR CHONDROCYTES CULTURED IN ALGINATE BEADS
    BONAVENTURE, J
    KADHOM, N
    COHENSOLAL, L
    NG, KH
    BOURGUIGNON, J
    LASSELIN, C
    FREISINGER, P
    [J]. EXPERIMENTAL CELL RESEARCH, 1994, 212 (01) : 97 - 104
  • [4] Buckwalter JA, 1998, ARTHRITIS RHEUM-US, V41, P1331, DOI 10.1002/1529-0131(199808)41:8<1331::AID-ART2>3.0.CO
  • [5] 2-J
  • [6] Bulpitt P, 1999, J BIOMED MATER RES, V47, P152
  • [7] Synthesis and physicochemical analysis of interpenetrating networks containing modified gelatin and poly(ethylene glycol) diacrylate
    Burmania, JA
    Martinez-Diaz, GJ
    Kao, WJ
    [J]. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, 2003, 67A (01): : 224 - 234
  • [8] Tissue engineering and cell therapy of cartilage and bone
    Cancedda, R
    Dozin, B
    Giannoni, P
    Quarto, R
    [J]. MATRIX BIOLOGY, 2003, 22 (01) : 81 - 91
  • [9] Scaffold-based articular cartilage repair
    Capito, RM
    Spector, M
    [J]. IEEE ENGINEERING IN MEDICINE AND BIOLOGY MAGAZINE, 2003, 22 (05): : 42 - 50
  • [10] Chondrogenic Differentiation of Adipose-Derived Adult Stem Cells by a Porous Scaffold Derived from Native Articular Cartilage Extracellular Matrix
    Cheng, Nai-Chen
    Estes, Bradley T.
    Awad, Hani A.
    Guilak, Farshid
    [J]. TISSUE ENGINEERING PART A, 2009, 15 (02) : 231 - 241
123456