Porous hyaluronic acid/sodium alginate composite scaffolds for human adipose-derived stem cells delivery

被引：18

作者：

Son, Yun-Jeong ^{[1
,2
]}

Yoon, In-Soo ^{[3
,4
]}

Sung, Jong-Hyuk ^{[5
]}

Cho, Hyun-Jong ^{[6
]}

Chung, Suk-Jae ^{[1
,2
]}

Shim, Chang-Koo ^{[1
,2
]}

Kim, Dae-Duk ^{[1
,2
]}

机构：

[1] Seoul Natl Univ, Coll Pharm, Seoul 151742, South Korea

[2] Seoul Natl Univ, Pharmaceut Sci Res Inst, Seoul 151742, South Korea

[3] Mokpo Natl Univ, Coll Pharm, Jeonnam 534729, South Korea

[4] Mokpo Natl Univ, Nat Med Res Inst, Jeonnam 534729, South Korea

[5] CHA Univ, Dept Appl Biosci, Seoul 135081, South Korea

[6] Kangwon Natl Univ, Coll Pharm, Chunchon 200701, South Korea

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2013年 / 61卷

基金：

新加坡国家研究基金会;

关键词：

Chondrogenic differentiation; Human adipose-derived stem cells; Hyaluronic acid; Interpenetrating polymeric network; Sodium alginate; INTERPENETRATING POLYMER NETWORKS; ARTICULAR-CARTILAGE REPAIR; CHONDROGENIC DIFFERENTIATION; ACID SCAFFOLD; BONE-MARROW; TISSUE; CHONDROCYTES; BEADS; TRANSPLANTATION; PHENOTYPE;

D O I：

10.1016/j.ijbiomac.2013.06.023

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The aim of this study is to evaluate the feasibility of hyaluronic acid/sodium alginate (HA/SA) scaffold-based interpenetrating polymeric network (IPN) for the proliferation and chondrogenic differentiation of the human adipose-derived stem cells (hADSCs). The hADSCs cultured in HA/SA IPN scaffold exhibited enhanced cell adhesion and proliferation compared to the HA scaffold. Superior chondrogenic differentiation of hADSCs in HA/SA IPN scaffold, compared to HA-based scaffold, was confirmed by measuring expression levels of chondrogenic markers. These results suggested that HA/SA IPN scaffold could provide a desirable environment for the cell adhesion, proliferation and chondrogenic differentiation of hADSCs. (C) 2013 Elsevier B.V. All rights reserved.

引用

页码：175 / 181

页数：7

共 53 条

[1]

Allemann M, 2001, J BIOMED MATER RES, V55, P13

[2] Isolation of human mesenchymal stem cells from bone and adipose tissue [J].

Bernacki, Susan H. ;

Wall, Michelle E. ;

Loboa, Elizabeth G. .

STEM CELL CULTURE, 2008, 86 :257-278

[3] REEXPRESSION OF CARTILAGE-SPECIFIC GENES BY DEDIFFERENTIATED HUMAN ARTICULAR CHONDROCYTES CULTURED IN ALGINATE BEADS [J].

BONAVENTURE, J ;

KADHOM, N ;

COHENSOLAL, L ;

NG, KH ;

BOURGUIGNON, J ;

LASSELIN, C ;

FREISINGER, P .

EXPERIMENTAL CELL RESEARCH, 1994, 212 (01) :97-104

[4]

Buckwalter JA, 1998, ARTHRITIS RHEUM-US, V41, P1331, DOI 10.1002/1529-0131(199808)41:8<1331::AID-ART2>3.0.CO

[5]

2-J

[6]

Bulpitt P, 1999, J BIOMED MATER RES, V47, P152

[7] Synthesis and physicochemical analysis of interpenetrating networks containing modified gelatin and poly(ethylene glycol) diacrylate [J].

Burmania, JA ;

Martinez-Diaz, GJ ;

Kao, WJ .

JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, 2003, 67A (01) :224-234

[8] Tissue engineering and cell therapy of cartilage and bone [J].

Cancedda, R ;

Dozin, B ;

Giannoni, P ;

Quarto, R .

MATRIX BIOLOGY, 2003, 22 (01) :81-91

[9] Scaffold-based articular cartilage repair [J].

Capito, RM ;

Spector, M .

IEEE ENGINEERING IN MEDICINE AND BIOLOGY MAGAZINE, 2003, 22 (05) :42-50

[10] Chondrogenic Differentiation of Adipose-Derived Adult Stem Cells by a Porous Scaffold Derived from Native Articular Cartilage Extracellular Matrix [J].

Cheng, Nai-Chen ;

Estes, Bradley T. ;

Awad, Hani A. ;

Guilak, Farshid .

TISSUE ENGINEERING PART A, 2009, 15 (02) :231-241

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