A genetically active nano-calcium phosphate paste for bone substitution, encoding the formation of BMP-7 and VEGF-A

被引：28
作者：
Chernousova, Svitlana
Klesing, Jan
Soklakova, Nadiia
Epple, Matthias [1 ]
机构：
[1] Univ Duisburg Essen, D-45117 Essen, Germany
来源：
RSC ADVANCES | 2013年 / 3卷 / 28期
关键词：
MESENCHYMAL STEM-CELLS; GENE DELIVERY; IN-VITRO; NANOCRYSTALLINE HYDROXYAPATITE; INORGANIC NANOPARTICLES; MORPHOGENETIC PROTEINS; POLYLACTIC ACID; NUCLEIC-ACIDS; TRANSFECTION; EXPRESSION;
D O I：
10.1039/c3ra23450a
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Calcium phosphate nanoparticles with different compositions and morphologies loaded with DNA were prepared. Plasmids which encoded either bone morphogenetic protein 7 (BMP-7) or vascular endothelial growth factor A (VEGF-A) were used for transfection in epithelial cells (HeLa), osteoblast-like cells (MG-63), and human mesenchymal stem cells (hMSC). In particular, cationic nanoparticles showed high transfection efficiency together with low cytotoxicity. The nanoparticles can either be used in dispersion or added to a calcium phosphate paste for injection into bone defects.
引用
收藏
页码：11155 / 11161
页数：7
相关论文
共 66 条
  • [1] The use of a bioresorbable nano-crystalline hydroxyapatite paste in acetabular bone impaction grafting
    Arts, JJC
    Verdonschot, N
    Schreurs, BW
    Buma, P
    [J]. BIOMATERIALS, 2006, 27 (07) : 1110 - 1118
  • [2] Osteogenic protein-1 (BMP-7) accelerates healing of scaphoid non-union with proximal pole sclerosis
    Bilic, R
    Simic, P
    Jelic, M
    Stern-Padovan, R
    Dodig, D
    van Meerdervoort, HP
    Martinovic, S
    Ivankovic, D
    Pecina, M
    Vukicevic, S
    [J]. INTERNATIONAL ORTHOPAEDICS, 2006, 30 (02) : 128 - 134
  • [3] Resorbable biomaterials as bone graft substitutes
    Bohner, Marc
    [J]. MATERIALS TODAY, 2010, 13 (1-2) : 24 - 30
  • [4] Gene-enhanced tissue engineering: Applications for bone healing using cultured periosteal cells transduced retrovirally with the BMP-7 gene
    Breitbart, AS
    Grande, DA
    Mason, JM
    Barcia, M
    James, T
    Grant, RT
    [J]. ANNALS OF PLASTIC SURGERY, 1999, 42 (05) : 488 - 495
  • [5] Cytotoxicity of polyethyleneimine (PEI), precursor base layer of polyelectrolyte multilayer films
    Brunot, Celine
    Ponsonnet, Laurence
    Lagneau, Christelle
    Farge, Pierre
    Picart, Catherine
    Grosgogeat, Brigitte
    [J]. BIOMATERIALS, 2007, 28 (04) : 632 - 640
  • [6] Simultaneous in vivo comparison of bone substitutes in a guided bone regeneration model
    Busenlechner, Dieter
    Tangl, Stefan
    Mair, Birgit
    Fugger, Georg
    Gruber, Reinhard
    Redl, Heinz
    Watzek, Georg
    [J]. BIOMATERIALS, 2008, 29 (22) : 3195 - 3200
  • [7] Calcium phosphate nanoparticles in biomineralization and biomaterials
    Cai, Yurong
    Tang, Ruikang
    [J]. JOURNAL OF MATERIALS CHEMISTRY, 2008, 18 (32) : 3775 - 3787
  • [8] Layer-By-Layer Films as a Biomimetic Reservoir for rhBMP-2 Delivery: Controlled Differentiation of Myoblasts to Osteoblasts
    Crouzier, Thomas
    Ren, Kefeng
    Nicolas, Claire
    Roy, Chrstian
    Picart, Catherine
    [J]. SMALL, 2009, 5 (05) : 598 - 608
  • [9] Expression of vascular endothelial growth factors and their receptors during osteoblast differentiation
    Deckers, MML
    Karperien, M
    van der Bent, C
    Yamashita, T
    Papapoulos, SE
    Löwik, CWGM
    [J]. ENDOCRINOLOGY, 2000, 141 (05) : 1667 - 1674
  • [10] Formation of osteoclast-like cells on HA and TCP ceramics
    Detsch, R.
    Mayr, H.
    Ziegler, G.
    [J]. ACTA BIOMATERIALIA, 2008, 4 (01) : 139 - 148
1234567