A genetically active nano-calcium phosphate paste for bone substitution, encoding the formation of BMP-7 and VEGF-A

被引：28
作者：
Chernousova, Svitlana
Klesing, Jan
Soklakova, Nadiia
Epple, Matthias [1 ]
机构：
[1] Univ Duisburg Essen, D-45117 Essen, Germany
来源：
RSC ADVANCES | 2013年 / 3卷 / 28期
关键词：
MESENCHYMAL STEM-CELLS; GENE DELIVERY; IN-VITRO; NANOCRYSTALLINE HYDROXYAPATITE; INORGANIC NANOPARTICLES; MORPHOGENETIC PROTEINS; POLYLACTIC ACID; NUCLEIC-ACIDS; TRANSFECTION; EXPRESSION;
D O I：
10.1039/c3ra23450a
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Calcium phosphate nanoparticles with different compositions and morphologies loaded with DNA were prepared. Plasmids which encoded either bone morphogenetic protein 7 (BMP-7) or vascular endothelial growth factor A (VEGF-A) were used for transfection in epithelial cells (HeLa), osteoblast-like cells (MG-63), and human mesenchymal stem cells (hMSC). In particular, cationic nanoparticles showed high transfection efficiency together with low cytotoxicity. The nanoparticles can either be used in dispersion or added to a calcium phosphate paste for injection into bone defects.
引用
收藏
页码：11155 / 11161
页数：7
相关论文
共 66 条
[1]   The use of a bioresorbable nano-crystalline hydroxyapatite paste in acetabular bone impaction grafting [J].
Arts, JJC ;
Verdonschot, N ;
Schreurs, BW ;
Buma, P .
BIOMATERIALS, 2006, 27 (07) :1110-1118
[2]   Osteogenic protein-1 (BMP-7) accelerates healing of scaphoid non-union with proximal pole sclerosis [J].
Bilic, R ;
Simic, P ;
Jelic, M ;
Stern-Padovan, R ;
Dodig, D ;
van Meerdervoort, HP ;
Martinovic, S ;
Ivankovic, D ;
Pecina, M ;
Vukicevic, S .
INTERNATIONAL ORTHOPAEDICS, 2006, 30 (02) :128-134
[3]   Resorbable biomaterials as bone graft substitutes [J].
Bohner, Marc .
MATERIALS TODAY, 2010, 13 (1-2) :24-30
[4]   Gene-enhanced tissue engineering: Applications for bone healing using cultured periosteal cells transduced retrovirally with the BMP-7 gene [J].
Breitbart, AS ;
Grande, DA ;
Mason, JM ;
Barcia, M ;
James, T ;
Grant, RT .
ANNALS OF PLASTIC SURGERY, 1999, 42 (05) :488-495
[5]   Cytotoxicity of polyethyleneimine (PEI), precursor base layer of polyelectrolyte multilayer films [J].
Brunot, Celine ;
Ponsonnet, Laurence ;
Lagneau, Christelle ;
Farge, Pierre ;
Picart, Catherine ;
Grosgogeat, Brigitte .
BIOMATERIALS, 2007, 28 (04) :632-640
[6]   Simultaneous in vivo comparison of bone substitutes in a guided bone regeneration model [J].
Busenlechner, Dieter ;
Tangl, Stefan ;
Mair, Birgit ;
Fugger, Georg ;
Gruber, Reinhard ;
Redl, Heinz ;
Watzek, Georg .
BIOMATERIALS, 2008, 29 (22) :3195-3200
[7]   Calcium phosphate nanoparticles in biomineralization and biomaterials [J].
Cai, Yurong ;
Tang, Ruikang .
JOURNAL OF MATERIALS CHEMISTRY, 2008, 18 (32) :3775-3787
[8]   Layer-By-Layer Films as a Biomimetic Reservoir for rhBMP-2 Delivery: Controlled Differentiation of Myoblasts to Osteoblasts [J].
Crouzier, Thomas ;
Ren, Kefeng ;
Nicolas, Claire ;
Roy, Chrstian ;
Picart, Catherine .
SMALL, 2009, 5 (05) :598-608
[9]   Expression of vascular endothelial growth factors and their receptors during osteoblast differentiation [J].
Deckers, MML ;
Karperien, M ;
van der Bent, C ;
Yamashita, T ;
Papapoulos, SE ;
Löwik, CWGM .
ENDOCRINOLOGY, 2000, 141 (05) :1667-1674
[10]   Formation of osteoclast-like cells on HA and TCP ceramics [J].
Detsch, R. ;
Mayr, H. ;
Ziegler, G. .
ACTA BIOMATERIALIA, 2008, 4 (01) :139-148
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