Biofilm disruption activity of silver nanoparticles synthesized by Acinetobacter calcoaceticus PUCM 1005

被引：52

作者：

Gaidhani, Sharvari ^{[1
]}

Singh, Richa ^{[2
]}

Singh, Divya ^{[1
]}

Patel, Urvashi ^{[1
]}

Shevade, Kaivalya ^{[1
]}

Yeshvekar, Richa ^{[1
]}

Chopade, Balu Ananda ^{[1
,2
]}

机构：

[1] Univ Pune, Inst Bioinformat & Biotechnol, Pune 411007, Maharashtra, India

[2] Univ Pune, Dept Microbiol, Pune 411007, Maharashtra, India

来源：

MATERIALS LETTERS | 2013年 / 108卷

关键词：

Acinetobacter calcoaceticus; Silver nanoparticles; Biomaterials; Electron microscopy; Antimicrobial activity; Biofilm disruption; BIOSYNTHESIS; RESISTANCE; EXTRACT;

D O I：

10.1016/j.matlet.2013.07.023

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

Biosynthesis of silver nanoparticles (AgNPs) using Acinetobacter calcoaceticus PUCM 1005 and their application in biofilm disruption is described. Extracellular AgNPs were produced by 48 h grown culture when challenged with silver nitrate. AgNPs synthesis was found to be optimum at 40 mM AgNO3, 40 degrees C temperature and pH 5. UV-vis spectroscopy, energy dispersive spectra and X-ray diffraction analysis confirmed presence of AgNPs. Electron microscopy revealed the size range to be 4-40 nm. These AgNPs exhibited higher activity against planktonic Gram negative pathogens. However, they could disrupt biofilm formed by Gram negative and Gram positive pathogenic microorganisms. AgNP synthesis was protein mediated. (C) 2013 Elsevier B.V. All rights reserved.

引用

页码：324 / 327

页数：4

共 23 条

[1]

Ahmad Naheed, 2011, Biotechnol Res Int, V2011, P454090, DOI 10.4061/2011/454090

[2]

[Anonymous], 2012, Nanoscience Methods, DOI DOI 10.1080/17458080.2010.529172

[3] Toward greener nanosynthesis [J].

Dahl, Jennifer A. ;

Maddux, Bettye L. S. ;

Hutchison, James E. .

CHEMICAL REVIEWS, 2007, 107 (06) :2228-2269

[4] Understanding biofilm resistance to antibacterial agents [J].

Davies, D .

NATURE REVIEWS DRUG DISCOVERY, 2003, 2 (02) :114-122

[5]

DESHPANDE LM, 1994, BIOMETALS, V7, P49

[6] Purification and Partial Characterization of Lignin Peroxidase from Acinetobacter calcoaceticus NCIM 2890 and Its Application in Decolorization of Textile Dyes [J].

Ghodake, Gajanan S. ;

Kalme, Satish D. ;

Jadhav, Jyoti P. ;

Govindwar, Sanjay P. .

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 2009, 152 (01) :6-14

[7] Gnidia glauca flower extract mediated synthesis of gold nanoparticles and evaluation of its chemocatalytic potential [J].

Ghosh, Sougata ;

Patil, Sumersing ;

Ahire, Mehul ;

Kitture, Rohini ;

Gurav, Deepanjali D. ;

Jabgunde, Amit M. ;

Kale, Sangeeta ;

Pardesi, Karishma ;

Shinde, Vaishali ;

Bellare, Jayesh ;

Dhavale, Dilip D. ;

Chopade, Balu A. .

JOURNAL OF NANOBIOTECHNOLOGY, 2012, 10

[8] Synthesis of silver nanoparticles using Dioscorea bulbifera tuber extract and evaluation of its synergistic potential in combination with antimicrobial agents [J].

Ghosh, Sougata ;

Patil, Sumersing ;

Ahire, Mehul ;

Kitture, Rohini ;

Kale, Sangeeta ;

Pardesi, Karishma ;

Cameotra, Swaranjit S. ;

Bellare, Jayesh ;

Dhavale, Dilip D. ;

Jabgunde, Amit ;

Chopade, Balu A. .

INTERNATIONAL JOURNAL OF NANOMEDICINE, 2012, 7 :483-496

[9] The clinical impact of bacterial biofilms [J].

Hoiby, Niels ;

Ciofu, Oana ;

Johansen, Helle Krogh ;

Song, Zhi-jun ;

Moser, Claus ;

Jensen, Peter Ostrup ;

Molin, Soren ;

Givskov, Michael ;

Tolker-Nielsen, Tim ;

Bjarnsholt, Thomas .

INTERNATIONAL JOURNAL OF ORAL SCIENCE, 2011, 3 (02) :55-65

[10] What drives bacteria to produce a biofilm? [J].

Jefferson, KK .

FEMS MICROBIOLOGY LETTERS, 2004, 236 (02) :163-173

← 1 2 3 →