Versatile PCR-mediated insertion or deletion mutagenesis

被引:83
作者
Lee, J [1 ]
Lee, HJ [1 ]
Shin, MK [1 ]
Ryu, WS [1 ]
机构
[1] Yonsei Univ, Seoul 120749, South Korea
关键词
D O I
10.2144/04363BM04
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
引用
收藏
页码:398 / +
页数:2
相关论文
共 6 条
[1]  
HORTON RM, 1993, METHOD ENZYMOL, V217, P270
[2]  
HORTON RM, 1990, BIOTECHNIQUES, V8, P528
[3]   Evidence that the 5′-end cap structure is essential for encapsidation of hepatitis B virus pregenomic RNA [J].
Jeong, JK ;
Yoon, GS ;
Ryu, WS .
JOURNAL OF VIROLOGY, 2000, 74 (12) :5502-5508
[4]   Rapid and efficient site-directed mutagenesis by single-tube 'megaprimer' PCR method [J].
Ke, SH ;
Madison, EL .
NUCLEIC ACIDS RESEARCH, 1997, 25 (16) :3371-3372
[5]   PCR-mediated generation of a gene disruption construct without the use of DNA ligase and plasmid vectors [J].
Kuwayama, H ;
Obara, S ;
Morio, T ;
Katoh, M ;
Urushihara, H ;
Tanaka, Y .
NUCLEIC ACIDS RESEARCH, 2002, 30 (02) :E2
[6]   EXPRESSION OF HEPATITIS-DELTA VIRUS-RNA DELETIONS - CIS AND TRANS REQUIREMENTS FOR SELF-CLEAVAGE, LIGATION, AND RNA PACKAGING [J].
LAZINSKI, DW ;
TAYLOR, JM .
JOURNAL OF VIROLOGY, 1994, 68 (05) :2879-2888