A light-emitting diode light source for imaging of neural activities with voltage-sensitive dyes

被引：14

作者：

Nishimura, M ^{[1
]}

Shirasawa, H ^{[1
]}

Song, WJ ^{[1
]}

机构：

[1] Osaka Univ, Grad Sch Engn, Dept Elect Engn, Suita, Osaka 5650871, Japan

来源：

NEUROSCIENCE RESEARCH | 2006年 / 54卷 / 03期

关键词：

optical recording; excitation light source; high-power LED; cortical activity;

D O I：

10.1016/j.neures.2005.12.002

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

We tested the possibility of using a high-power monochromatic InGaN light-emitting diode (LED) as an excitation light source for real-time optical imaging using the voltage-sensitive dye RH-795. Driven with a custom-designed, non-feedback-controlled constant-current circuit, the LED generated stable light with rapid on/off. The LED was comparable with commonly used halogen lamps in exciting RH-795. Acoustically evoked responses in the auditory cortex recorded with the two light sources were highly similar. Our results thus suggest that a high-power LED can be successfully used as an excitation light source for voltage-sensitive dyes, without the need of optical filters and shutters. (c) 2005 Elsevier Ireland Ltd and the Japan Neuroscience Society. All rights reserved.

引用

页码：230 / 234

页数：5

共 16 条

[1] OPTICAL METHODS FOR MONITORING NEURON ACTIVITY [J].

COHEN, LB ;

SALZBERG, BM ;

GRINVALD, A .

ANNUAL REVIEW OF NEUROSCIENCE, 1978, 1 :171-182

[2]

GRINVALD A, 1994, J NEUROSCI, V14, P2545

[3] FLUORESCENCE MONITORING OF ELECTRICAL RESPONSES FROM SMALL NEURONS AND THEIR PROCESSES [J].

GRINVALD, A ;

FINE, A ;

FARBER, IC ;

HILDESHEIM, R .

BIOPHYSICAL JOURNAL, 1983, 42 (02) :195-198

[4]

Grinvald A., 1999, MODERN TECHNIQUES NE, P893

[5] Optical and FDG mapping of frequency-specific activity in auditory cortex [J].

Hess, A ;

Scheich, H .

NEUROREPORT, 1996, 7 (15-17) :2643-2647

[6] Isolation of neural activities from respiratory and heartbeat noises for in vivo optical recording in guinea pigs using independent component analysis [J].

Inagaki, S ;

Katura, T ;

Kawaguchi, H ;

Song, WJ .

NEUROSCIENCE LETTERS, 2003, 352 (01) :9-12

[7] OPTICAL APPROACHES TO ONTOGENY OF ELECTRICAL-ACTIVITY AND RELATED FUNCTIONAL-ORGANIZATION DURING EARLY HEART DEVELOPMENT [J].

KAMINO, K .

PHYSIOLOGICAL REVIEWS, 1991, 71 (01) :53-91

[8] Optical mapping of the functional organization of the rat trigeminal nucleus: Initial expression and spatiotemporal dynamics of sensory information transfer during embryogenesis [J].

Momose-Sato, Y ;

Honda, Y ;

Sasaki, H ;

Sato, K .

JOURNAL OF NEUROSCIENCE, 2004, 24 (06) :1366-1376

[9] FUNCTIONAL SUBDIVISIONS IN THE AUDITORY-CORTEX OF THE GUINEA-PIG [J].

REDIES, H ;

SIEBEN, U ;

CREUTZFELDT, OD .

JOURNAL OF COMPARATIVE NEUROLOGY, 1989, 282 (04) :473-488

[10] Low frequency noise and long-term stability of noncoherent light sources [J].

Rumyantsev, SL ;

Shur, MS ;

Bilenko, Y ;

Kosterin, PV ;

Salzberg, BM .

JOURNAL OF APPLIED PHYSICS, 2004, 96 (02) :966-969

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